Gene Expression Data
Assay Details
Assay
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Reference:
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J:69787
Kim AS, et al., Wnt receptors and Wnt inhibitors are expressed in gradients in the developing telencephalon. Mech Dev. 2001 May;103(1-2):167-72
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2675378
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Gene symbol:
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Sfrp1
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Gene name:
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secreted frizzled-related protein 1
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Probe:
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Sfrp1 cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
1A:
embryonic day 10.5
(more )
Note: Vibratome section of a whole mount-stained specimen.
Specimen
1A:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 10.5
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Age Note:
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Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Vibratome section of a whole mount-stained specimen.
|
Structure |
Level |
Pattern |
Image |
Note |
TS18: telencephalon |
Present |
Regionally restricted |
1A
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Expression is excluded at the most medial region of the cortical neuroepithelium.
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Specimen
1D:
embryonic day 12.5
(more )
Note: Vibratome section of a whole mount-stained specimen.
Specimen
1D:
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Vibratome section of a whole mount-stained specimen.
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: telencephalon |
Present |
Graded |
1D
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Expression is in an anterior-high to posterior-low gradient.
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Specimen
3D:
embryonic day 14.5
(more )
Specimen
3D:
(close )
Genetic Background:
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CD-1
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: neocortex |
Present |
Regionally restricted |
3D
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Expression is in the lateral neocortical neuroepithelium, and the dorsal portion of the hippocampal neuorepithelium.
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TS22: lateral ganglionic eminence |
Present |
|
3D
|
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TS22: hippocampus |
Present |
Regionally restricted |
3D
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Expression is in the dorsal portion of the hippocampal neuorepithelium.
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Specimen
3D':
embryonic day 14.5
(more )
Specimen
3D':
(close )
Genetic Background:
|
CD-1
|
Age:
|
embryonic day 14.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
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Specimen
4B:
embryonic day 17.5
(more )
Specimen
4B:
(close )
Genetic Background:
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CD-1
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Age:
|
embryonic day 17.5
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: cerebral cortex |
Present |
|
4B
|
|
TS25: diencephalon |
Present |
|
4B
|
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TS25: telencephalon |
Present |
Graded |
4B
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Expression is in an high-lateral to low-medial gradient in the ependymal layer and is absent in the more ventral structures along the medial neuroepithelium or in the fimbrial neuorepithelium.
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