Gene Expression Data
Assay Details
Assay
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Reference:
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J:85472
Kawahira H, et al., Combined activities of hedgehog signaling inhibitors regulate pancreas development. Development. 2003 Oct;130(20):4871-9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2675752
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Gene symbol:
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Isl1
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Gene name:
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ISL1 transcription factor, LIM/homeodomain
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Antibody:
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anti-Islet-1
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Detection system:
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Secondary antibody coupled to biotin
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Results
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Specimen
6H:
embryonic day 18.5; Ptch1tm1Mps/Ptch1 +
(more )
Specimen
6H:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ptch1tm1Mps/Ptch1+
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS26: pancreas |
Present |
Regionally restricted |
6H
|
|
Specimen
6I:
(close )
Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Hhiptm1Amc/Hhiptm1Amc
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Age:
|
embryonic day 18.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: pancreas |
Present |
Regionally restricted |
6I
|
|
Specimen
6J:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Hhiptm1Amc/Hhiptm1Amc Ptch1tm1Mps/Ptch1+
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Age:
|
embryonic day 18.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: pancreas |
Present |
Regionally restricted |
6J
|
|
Specimen
7A:
embryonic day 12.5; Ptch1tm1Mps/Ptch1 +
(more )
Specimen
7A:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
Mutant Allele(s):
|
Ptch1tm1Mps/Ptch1+
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: stomach |
Strong |
Regionally restricted |
7A
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Expression in posterior stomach mesenchyme.
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Specimen
7B:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
|
Mutant Allele(s):
|
Hhiptm1Amc/Hhiptm1Amc
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: stomach |
Present |
Regionally restricted |
7B
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Expression in posterior stomach mesenchyme is reduced.
|
Specimen
7C:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
|
Hhiptm1Amc/Hhiptm1Amc Ptch1tm1Mps/Ptch1+
|
Age:
|
embryonic day 12.5
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: stomach |
Weak |
Regionally restricted |
7C
|
Reduction of expression in posterior stomach mesenchyme is even more pronounced.
|
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