Gene Expression Data
Assay Details
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Assay
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Reference:
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J:85473
Corbin JG, et al., Combinatorial function of the homeodomain proteins Nkx2.1 and Gsh2 in ventral telencephalic patterning. Development. 2003 Oct;130(20):4895-906
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:2676233
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Gene symbol:
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Nkx2-1
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Gene name:
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NK2 homeobox 1
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Results
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Specimen
1A,1C:
embryonic day 9.5
(more  )
Note: 23 somites. Triple labeled: red - Nkx2-1; green - Pax6 (assay MGI:2676225); blue - Shh (assay MGI:2676237).
Specimen
1A,1C:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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23 somites. Triple labeled: red - Nkx2-1; green - Pax6 (assay MGI:2676225); blue - Shh (assay MGI:2676237).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: telencephalon |
Present |
Regionally restricted |
1A
,
1C
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Expression of Pax6 and Nkx2.1 forms a boundary in the ventral telencephalon.
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Specimen
1D,1F:
embryonic day 10.0
(more )
Note: 27 somites. Triple labeled: red - Nkx2-1; green - Pax6 (assay MGI:2676225); blue - Gsx2 (assay MGI:2676238).
Specimen
1D,1F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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27 somites. Triple labeled: red - Nkx2-1; green - Pax6 (assay MGI:2676225); blue - Gsx2 (assay MGI:2676238).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: telencephalon |
Present |
Regionally restricted |
1F
,
1D
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Slight gap between Pax6 and Nkx2.1 expression.
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Specimen
1G,1I:
embryonic day 10.5
(more )
Note: Triple labeled: red - Nkx2-1; green - Pax6 (assay MGI:2676225); blue - Gsx2 (assay MGI:2676238).
Specimen
1G,1I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Triple labeled: red - Nkx2-1; green - Pax6 (assay MGI:2676225); blue - Gsx2 (assay MGI:2676238).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: telencephalon |
Present |
Regionally restricted |
1I
,
1G
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Gap in expression of Pax6 and Nkx2.1 expands.
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Specimen
2A:
embryonic day 10.5
(more )
Note: 36-38 somites. Double labeled: red - Nkx2-1; green - Gsx2 (assay MGI:2676238).
Specimen
2A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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36-38 somites. Double labeled: red - Nkx2-1; green - Gsx2 (assay MGI:2676238).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: telencephalon |
Present |
Regionally restricted |
2A
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Not expressed in anterior telencephalon.
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Specimen
2B:
embryonic day 10.5
(more )
Note: 36-38 somites. Double labeled: red - Nkx2-1; green - Gsx2 (assay MGI:2676238).
Specimen
2B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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36-38 somites. Double labeled: red - Nkx2-1; green - Gsx2 (assay MGI:2676238).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: telencephalon |
Present |
Regionally restricted |
2B
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The most anterior expression is at the level of the septum.
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Specimen
2C:
embryonic day 10.5
(more )
Note: 36-38 somites. Double labeled: red - Nkx2-1; green - Gsx2 (assay MGI:2676238).
Specimen
2C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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36-38 somites. Double labeled: red - Nkx2-1; green - Gsx2 (assay MGI:2676238).
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Specimen
2D:
embryonic day 10.5
(more )
Note: 36-38 somites. Double labeled: red - Nkx2-1; green - Gsx2 (assay MGI:2676238).
Specimen
2D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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36-38 somites. Double labeled: red - Nkx2-1; green - Gsx2 (assay MGI:2676238).
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Specimen
2E:
embryonic day 10.5
(more )
Note: 36-38 somites. Double labeled: red - Nkx2-1; green - Gsx2 (assay MGI:2676238).
Specimen
2E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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36-38 somites. Double labeled: red - Nkx2-1; green - Gsx2 (assay MGI:2676238).
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Specimen
2F:
embryonic day 10.5
(more )
Note: 36-38 somites. Double labeled: red - Nkx2-1; green - Gsx2 (assay MGI:2676238).
Specimen
2F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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36-38 somites. Double labeled: red - Nkx2-1; green - Gsx2 (assay MGI:2676238).
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Specimen
3A:
embryonic day 9.5; Pax6Sey/Pax6 +
(more )
Note: 22 somites.
Specimen
3A:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Pax6Sey/Pax6+
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Age:
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embryonic day 9.5
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Age Note:
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Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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22 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: telencephalon |
Present |
Regionally restricted |
3A
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Expression of Pax6 and Nkx2.1 form a distinct border.
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Specimen
3B:
embryonic day 9.5; Pax6Sey/ Pax6Sey
(more )
Note: 22 somites.
Specimen
3B:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 9.5
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Age Note:
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Age was assigned based on somite numbers reported in the reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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22 somites.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: telencephalon |
Present |
Regionally restricted |
3B
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Expression does not expand dorsally.
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Specimen
3G:
embryonic day 10.0; Gsx2tm1Ssp/Gsx2 +
(more )
Note: 31-32 somites.
Specimen
3G:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Gsx2tm1Ssp/Gsx2+
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Age:
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embryonic day 10.0
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Age Note:
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Age was assigned based on somite numbers reported in the reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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31-32 somites.
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Specimen
3H:
embryonic day 10.0; Pax6Sey/ Pax6Sey
(more )
Note: 31-32 somites.
Specimen
3H:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 10.0
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Age Note:
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Age was assigned based on somite numbers reported in the reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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31-32 somites.
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Specimen
S1 A:
embryonic day 9.5
(more )
Note: Double labeled: green - Nkx2-1; red - Shh (assay MGI:2676237).
Specimen
S1 A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Nkx2-1; red - Shh (assay MGI:2676237).
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Specimen
S1 B:
(close )
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Genetic Background:
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involves: 129S4/SvJae
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Mutant Allele(s):
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Nkx2-1tm1Shk/Nkx2-1tm1Shk
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Nkx2-1; red - Shh (assay MGI:2676237).
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