Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:79391
Fujiwara T, et al., Distinct requirements for extra-embryonic and embryonic bone morphogenetic protein 4 in the formation of the node and primitive streak and coordination of left-right asymmetry in the mouse. Development. 2002 Oct;129(20):4685-96
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:2676359
|
|
Gene symbol:
|
Fgf4
|
|
Gene name:
|
fibroblast growth factor 4
|
|
Probe:
|
Fgf4 probe14
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
2D:
embryonic day 8.0
(more  )
Note: 1 somite stage.
Specimen
2D:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age was assigned based on somite numbers reported in the reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
1 somite stage.
|
Specimen
2E:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * 129S6/SvEvTac * Black Swiss
|
|
Mutant Allele(s):
|
Bmp4tm1Blh/Bmp4tm1Blh
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age was assigned based on somite numbers reported in the reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
1 somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: primitive streak |
Present |
|
2E
|
Comparatively normal primitive streak expression in 1/4 mutant embryos.
|
Specimen
2F:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * 129S6/SvEvTac * Black Swiss
|
|
Mutant Allele(s):
|
Bmp4tm1Blh/Bmp4tm1Blh
|
|
Age:
|
embryonic day 8.0
|
|
Age Note:
|
Age was assigned based on somite numbers reported in the reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
1 somite stage.
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS12: embryo |
Weak |
Regionally restricted |
2F
|
Expression strongly reduced in posterior of 3/4 mutant embryos.
|
|
Analysis Tools
