Gene Expression Data
Assay Details
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Assay
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Reference:
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J:79394
Ishibashi M, et al., A sonic hedgehog-dependent signaling relay regulates growth of diencephalic and mesencephalic primordia in the early mouse embryo. Development. 2002 Oct;129(20):4807-19
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2676606
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Gene symbol:
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En1
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Gene name:
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engrailed 1
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Probe:
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En1 probe6
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1E:
embryonic day 9.0
(more  )
Note: 14 somite stage.
Specimen
1E:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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14 somite stage.
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Specimen
1F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Shhtm1Amc/Shhtm1Amc
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Age:
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embryonic day 9.0
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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14 somite stage.
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Specimen
1G:
embryonic day 9.5
(more )
Note: 21 somite stage.
Specimen
1G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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21 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future midbrain |
Present |
Regionally restricted |
1G
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Expression downregulated in anterior midbrain.
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Specimen
1H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Shhtm1Amc/Shhtm1Amc
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Age:
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embryonic day 9.5
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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21 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future midbrain |
Present |
Regionally restricted |
1H
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Expression domain similar to wild-type. Gap between Emx2 and En1 domains is reduced
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Specimen
1I:
embryonic day 9.5
(more )
Note: 21 somite stage.
Specimen
1I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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21 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future midbrain |
Present |
Regionally restricted |
1I
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Expression downregulated in anterior midbrain.
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Specimen
1J:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Shhtm1Amc/Shhtm1Amc
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Age:
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embryonic day 9.5
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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21 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future midbrain |
Present |
Regionally restricted |
1J
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Size of expression domain not altered compared to wild-type. Gap between En1 and Pax6 domains is reduced.
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Analysis Tools