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Gene Expression Data
Assay Details
Assay
Reference: J:79394 Ishibashi M, et al., A sonic hedgehog-dependent signaling relay regulates growth of diencephalic and mesencephalic primordia in the early mouse embryo. Development. 2002 Oct;129(20):4807-19
Assay type: RNA in situ
MGI Accession ID: MGI:2676607
Gene symbol: Pax6
Gene name: paired box 6
Probe: Pax probe12
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 1I: embryonic day 9.5 (more )
Note: 21 somite stage.
Structure Level Pattern Image Note
TS15: diencephalon roof plate Present 1I Expressed throughout alar plate of forebrain; sharp posterior boundary demarcates the diencephalic-midbrain junction.
TS15: telencephalon roof plate Present 1I Expressed throughout alar plate of forebrain; sharp posterior boundary demarcates the diencephalic-midbrain junction.
TS15: future midbrain Absent 1I

Specimen 1J: embryonic day 9.5; Shhtm1Amc/Shhtm1Amc (more )
Note: 21 somite stage.
Structure Level Pattern Image Note
TS15: diencephalon roof plate Present 1J Marked decrease in size of expression domain in the forebrain. Gap between Pax6 and En1 domains is reduced.
TS15: telencephalon roof plate Present 1J Marked decrease in size of expression domain in the forebrain. Gap between Pax6 and En1 domains is reduced.

Specimen 2E: embryonic day 9.5 (more )
Note: 21 somite stage.
Structure Level Pattern Image Note
TS15: diencephalon roof plate Present 2E Expression in the forebrain terminates at the forebrain-midbrain boundary.
TS15: telencephalon roof plate Present 2E Expression in the forebrain terminates at the forebrain-midbrain boundary.
TS15: future midbrain Absent 2E

Specimen 2F: embryonic day 9.5; Shhtm1Amc/Shhtm1Amc (more )
Note: 21 somite stage.
Structure Level Pattern Image Note
TS15: diencephalon roof plate Present 2F Expressed in a simlar pattern and at a comparable level with wild type.
TS15: telencephalon roof plate Present 2F Expressed in a simlar pattern and at a comparable level with wild type.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory