Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:79394
Ishibashi M, et al., A sonic hedgehog-dependent signaling relay regulates growth of diencephalic and mesencephalic primordia in the early mouse embryo. Development. 2002 Oct;129(20):4807-19
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:2676607
|
Gene symbol:
|
Pax6
|
Gene name:
|
paired box 6
|
Probe:
|
Pax probe12
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
|
Results
|
Specimen
1I:
embryonic day 9.5
(more )
Note: 21 somite stage.
Specimen
1I:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Embryonic age was assigned based on somite numbers reported in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
21 somite stage.
|
Specimen
1J:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * Black Swiss
|
Mutant Allele(s):
|
Shhtm1Amc/Shhtm1Amc
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Embryonic age was assigned based on somite numbers reported in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
21 somite stage.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: diencephalon roof plate |
Present |
|
1J
|
Marked decrease in size of expression domain in the forebrain. Gap between Pax6 and En1 domains is reduced.
|
TS15: telencephalon roof plate |
Present |
|
1J
|
Marked decrease in size of expression domain in the forebrain. Gap between Pax6 and En1 domains is reduced.
|
Specimen
2E:
embryonic day 9.5
(more )
Note: 21 somite stage.
Specimen
2E:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Embryonic age was assigned based on somite numbers reported in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
21 somite stage.
|
Specimen
2F:
(close )
Genetic Background:
|
involves: 129S1/Sv * 129X1/SvJ * Black Swiss
|
Mutant Allele(s):
|
Shhtm1Amc/Shhtm1Amc
|
Age:
|
embryonic day 9.5
|
Age Note:
|
Embryonic age was assigned based on somite numbers reported in reference.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
21 somite stage.
|
|