Gene Expression Data
Assay Details
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Assay
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Reference:
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J:79394
Ishibashi M, et al., A sonic hedgehog-dependent signaling relay regulates growth of diencephalic and mesencephalic primordia in the early mouse embryo. Development. 2002 Oct;129(20):4807-19
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2676614
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Gene symbol:
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Tcf7l2
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Gene name:
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transcription factor 7 like 2, T cell specific, HMG box
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Probe:
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Tcf4 probe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
4A:
embryonic day 8.5
(more  )
Note: 12 somite stage.
Specimen
4A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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12 somite stage.
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Specimen
4B:
embryonic day 9.0
(more )
Note: 14 somite stage.
Specimen
4B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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14 somite stage.
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Specimen
4C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Shhtm1Amc/Shhtm1Amc
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Age:
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embryonic day 9.0
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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14 somite stage.
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Specimen
4D:
embryonic day 9.0
(more )
Note: 16 somite stage.
Specimen
4D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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16 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future diencephalon |
Strong |
Regionally restricted |
4D
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Expression levels in diencephalon increased.
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Specimen
4E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Shhtm1Amc/Shhtm1Amc
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Age:
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embryonic day 9.0
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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16 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future diencephalon |
Weak |
Regionally restricted |
4E
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Expression barely detectable in the dorsal diencephalic region.
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Analysis Tools