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Gene Expression Data
Assay Details
Assay
Reference: J:45695 Duvillie B, et al., Imprinting at the mouse Ins2 locus: evidence for cis- and trans-allelic interactions. Genomics. 1998 Jan 1;47(1):52-7
Assay type: RT-PCR
MGI Accession ID: MGI:2682267
Gene symbol: Ins2
Gene name: insulin II
Probe: Ins2-pI, Ins1/2-pD
Assay notes: The Ins2 RT-PCR product was differentiated from the Ins1 product by MspI digestion followed by Southern blot analysis using 5'-32P-labeled oligonucleotide 5'-GTCCTGATAGCGGTCCGCCA-3' as probe. These data are reported in Table 1.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
wt/wt E12.5 (a) TS20: yolk sac Present (b) 2.5 µg involves: C57BL/6 * DBA/2 Pooled
wt/wt E14.5 (a) TS22: yolk sac Present (c) 2.5 µg involves: C57BL/6 * DBA/2 Pooled
wt/wt E12.5 (a) TS20: embryo Present (b) 2.5 µg involves: C57BL/6 * DBA/2 Pooled
wt/wt E14.5 (a) TS22: embryo Present (b) 2.5 µg involves: C57BL/6 * DBA/2 Pooled
Zneo/wt E12.5 (a) TS20: yolk sac Present (b) 2.5 µg involves: 129S2/SvPas * C57BL/6 * DBA/2 Ins2tm1Jja/Ins2+Pooled
Zneo/wt E14.5 (a) TS22: yolk sac Present (c) 2.5 µg involves: 129S2/SvPas * C57BL/6 * DBA/2 Ins2tm1Jja/Ins2+Pooled
Zneo/wt E12.5 (a) TS20: embryo Present (b) 2.5 µg involves: 129S2/SvPas * C57BL/6 * DBA/2 Ins2tm1Jja/Ins2+Pooled
Zneo/wt E14.5 (a) TS22: embryo Present (b) 2.5 µg involves: 129S2/SvPas * C57BL/6 * DBA/2 Ins2tm1Jja/Ins2+Pooled
Neo/wt E12.5 (a) TS20: yolk sac Present (c) 2.5 µg involves: 129S2/SvPas * C57BL/6 * DBA/2 Ins2tm2Jja/Ins2+Pooled
Neo/wt E14.5 (a) TS22: yolk sac Present (c) 2.5 µg involves: 129S2/SvPas * C57BL/6 * DBA/2 Ins2tm2Jja/Ins2+Pooled
Neo/wt E12.5 (a) TS20: embryo Present (b) 2.5 µg involves: 129S2/SvPas * C57BL/6 * DBA/2 Ins2tm2Jja/Ins2+Pooled
Neo/wt E14.5 (a) TS22: embryo Present (b) 2.5 µg involves: 129S2/SvPas * C57BL/6 * DBA/2 Ins2tm2Jja/Ins2+Pooled
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Paternal and maternal expression.
(c) Paternal expression.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/17/2024
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