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Gene Expression Data
Assay Details
Assay
Reference: J:23776 Collins JE, et al., Regulation of desmocollin transcription in mouse preimplantation embryos. Development. 1995 Mar;121(3):743-53
Assay type: RT-PCR
MGI Accession ID: MGI:2683124
Gene symbol: Dsc2
Gene name: desmocollin 2
Probe: Primer 2, Primer 3
Visualized with: Ethidium bromide
Assay notes: The RT-PCR assay used nested primers to amplify from the product of Primers 1 and 4 (MGI:2683097).
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure 532 bp Amount Genetic BackgroundMutant Allele(s)Sex Note
a Mol. Wt. Marker Lane
b E2.0 (a) TS3: 4-cell stage conceptus Present Not Specified; poly-A+ RNA Not Specified Not Specified 4-cell embryo.
c E2.5 (a) TS3: 8-cell stage conceptus Not Specified (b) Not Specified; poly-A+ RNA Not Specified Not Specified pre-compact 8-cell embryo.
d E2.5 (a) TS3: 8-cell stage conceptus Not Specified (b) Not Specified; poly-A+ RNA Not Specified Not Specified pre-compact 8-cell embryo.
e E2.5 (a) TS3: compacted morula Absent Not Specified; poly-A+ RNA Not Specified Not Specified compact 8-cell embryo.
f E2.5 (a) TS3: compacted morula Absent Not Specified; poly-A+ RNA Not Specified Not Specified compact 8-cell embryo.
g E3.0 (a) TS4: compacted morula Not Specified (c) Not Specified; poly-A+ RNA Not Specified Not Specified 16-cell embryo.
h E3.0 (a) TS4: compacted morula Not Specified (c) Not Specified; poly-A+ RNA Not Specified Not Specified 16-cell embryo.
i E3.0 (a) TS4: compacted morula Not Specified (c) Not Specified; poly-A+ RNA Not Specified Not Specified 16-cell embryo.
j E3.0 (a) TS4: compacted morula Not Specified (c) Not Specified; poly-A+ RNA Not Specified Not Specified 16-cell embryo.
k E3.0 (a) TS4: compacted morula Not Specified (c) Not Specified; poly-A+ RNA Not Specified Not Specified 16-cell embryo.
l E3.0 (a) TS4: compacted morula Not Specified (c) Not Specified; poly-A+ RNA Not Specified Not Specified 16-cell embryo.
m E3.5 (a) TS4: blastocyst-stage conceptus Present Not Specified; poly-A+ RNA Not Specified Not Specified early blastocyst.
n Control
o Lane not used in this assay
p Control
q E4.0 (a) TS5: blastocyst-stage conceptus Present Not Specified; poly-A+ RNA Not Specified Not Specified early blastocyst.
r Control
s Mol. Wt. Marker Lane
Notes:
(a) Age of embryos is based on cell number in pre-implantation embryos.
(b) Expression was detected in 41% of pre-compact 8-cell embryos.
(c) Expression was detected in 46% of 16-cell embryos.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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09/17/2024
MGI 6.24 		The Jackson Laboratory