Gene Expression Data
Assay Details
Assay
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Reference:
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J:73955
Kobayashi S, et al., Paternal expression of a novel imprinted gene, Peg12/Frat3, in the mouse 7C region homologous to the Prader-Willi syndrome region. Biochem Biophys Res Commun. 2002 Jan 11;290(1):403-8
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:2686694
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Gene symbol:
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Peg12
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Gene name:
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paternally expressed 12
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Probe:
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Peg12-pA, Peg12-pB
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Visualized with:
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Ethidium bromide
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Assay notes:
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The PCR product amplified contained no MboII restriction site C57BL/6 (521bp product) and one site for JF1 (338 and 177bp products). The C57BL/6 and JF1 alleles could be distinguished by digestion with MboII. This assay indicates paternal expression, except day 10 placenta.
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Results
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Image: 3B
Control |
Control |
Control |
P adult
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TS28: spleen
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Absent
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Present
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1 µg; total RNA |
involves: C57BL/6 * JF1 |
| Not Specified |
P adult
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TS28: lung
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Absent
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Present
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1 µg; total RNA |
involves: C57BL/6 * JF1 |
| Not Specified |
P3
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TS27: telencephalon
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Absent
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Present
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1 µg; total RNA |
involves: C57BL/6 * JF1 |
| Not Specified |
P3
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TS27: cerebellum
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Absent
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Present
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1 µg; total RNA |
involves: C57BL/6 * JF1 |
| Not Specified |
E13.0
(c)
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TS21: embryo
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Absent
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Present
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1 µg; total RNA |
involves: C57BL/6 * JF1 |
| Not Specified |
E10.0
(c)
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TS16: extraembryonic component
TS28: placenta
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Present
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Present
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1 µg; total RNA |
involves: C57BL/6 * JF1 |
| Not Specified |
E10.0
(c)
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TS16: embryo
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Absent
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Present
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1 µg; total RNA |
involves: C57BL/6 * JF1 |
| Not Specified |
Notes:
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