Gene Expression Data
Assay Details
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Assay
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Reference:
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J:47699
Pabst O, et al., Nkx2-9 is a novel homeobox transcription factor which demarcates ventral domains in the developing mouse CNS. Mech Dev. 1998 Apr;73(1):85-93
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3032440
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Gene symbol:
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Shh
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Gene name:
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sonic hedgehog
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Probe:
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Shh cDNA12
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
4H:
embryonic day 8.0
(more  )
Note: 4 somites. Double labeled: red - Shh; blue - Nkx2-9 (assay MGI:3029967).
Specimen
4H:
(close  )
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Genetic Background:
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ICR
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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4 somites. Double labeled: red - Shh; blue - Nkx2-9 (assay MGI:3029967).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: future midbrain |
Present |
Regionally restricted |
4H
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Expression is in a domain directly adjacent to the expression domain of Nkx2-9.
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| TS12: future prosencephalon |
Present |
Regionally restricted |
4H
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Expression is in a domain directly adjacent to the expression domain of Nkx2-9.
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Specimen
4I:
embryonic day 8.0
(more )
Note: 4 somites. Double labeled: red - Shh; blue - Nkx2-9 (assay MGI:3029967).
Specimen
4I:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 8.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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4 somites. Double labeled: red - Shh; blue - Nkx2-9 (assay MGI:3029967).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: neural tube |
Present |
Regionally restricted |
4I
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Expression is in a domain directly adjacent to the expression domain of Nkx2-9.
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Specimen
4J:
embryonic day 8.5
(more )
Note: 10 somites. Double labeled: red - Shh; blue - Nkx2-9 (assay MGI:3029967).
Specimen
4J:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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10 somites. Double labeled: red - Shh; blue - Nkx2-9 (assay MGI:3029967).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future hindbrain |
Present |
Regionally restricted |
4J
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Expression is in a domain directly adjacent to the expression domain of Nkx2-9.
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| TS13: future prosencephalon |
Present |
Regionally restricted |
4J
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Expression is in a domain and there is partial overlap in expression with Nkx2-9.
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Specimen
4K:
embryonic day 8.5
(more )
Note: 10 somites. Double labeled: red - Shh; blue - Nkx2-9 (assay MGI:3029967).
Specimen
4K:
(close )
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Genetic Background:
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ICR
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Age:
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embryonic day 8.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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10 somites. Double labeled: red - Shh; blue - Nkx2-9 (assay MGI:3029967).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: neural tube |
Present |
Regionally restricted |
4K
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Expression is in a domain close to the expression domain of Nkx2-9.
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Analysis Tools