Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:17145
Sandell LJ, et al., Alternative splice form of type II procollagen mRNA (IIA) is predominant in skeletal precursors and non-cartilaginous tissues during early mouse development. Dev Dyn. 1994 Feb;199(2):129-40
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:3041848
|
Gene symbol:
|
Col2a1
|
Gene name:
|
collagen, type II, alpha 1
|
Probe:
|
Collagen IIA probe1
|
Probe preparation:
|
Antisense labelled with S35 DNA
|
Visualized with:
|
Autoradiography
|
Assay notes:
|
Identical results were obtained using another oligonucleotide probe (MGI:3041979). The probe used in this assay was 3' labelled.
|
|
Results
|
Specimen
1C:
embryonic day 15.0
(more )
Specimen
1C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 15.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: humerus |
Present |
Regionally restricted |
1C
|
Expression was detected in the prechondrocytes of cartilage anlage.
|
TS23: scapula |
Present |
Regionally restricted |
1C
|
Expression was detected in the prechondrocytes of cartilage anlage.
|
TS23: upper arm rest of mesenchyme |
Absent |
|
1C
|
Expression was absent from the tendon.
|
Specimen
2C:
embryonic day 11.0
(more )
Specimen
2C:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Specimen
3B:
embryonic day 11.0
(more )
Specimen
3B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Specimen
4B:
embryonic day 11.0
(more )
Specimen
4B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 11.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Specimen
5B:
embryonic day 18.0
(more )
Specimen
5B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Specimen
6B:
embryonic day 18.0
(more )
Specimen
6B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: labyrinth epithelium |
Present |
|
6A/B
|
Expression was detected in the epithelial cells of the membranous labyrinth.
|
TS26: otic cartilage |
Present |
Regionally restricted |
6A/B
|
Expression was detected in the chondroprogenitor cells adjacent to the cartilage of the osseus labyrinth primordium.
|
Specimen
7B:
embryonic day 18.0
(more )
Specimen
7B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: C3 vertebra |
Present |
Regionally restricted |
7A/B
|
Expression was detected in the chondroprogenitor cells.
|
TS26: C4 vertebra |
Present |
Regionally restricted |
7A/B
|
Expression was detected in the chondroprogenitor cells.
|
TS26: C5 vertebra |
Present |
Regionally restricted |
7A/B
|
Expression was detected in the chondroprogenitor cells.
|
Specimen
8B:
embryonic day 18.0
(more )
Specimen
8B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Specimen
9B:
embryonic day 18.0
(more )
Specimen
9B:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: mandible |
Present |
Regionally restricted |
9A/B
|
Expression was detected in the mesenchymal cells.
|
|