Probe:	Bek probe
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS28: ovary	Weak	Regionally restricted		Expression is present in the ovarian stroma.
TS28: ovary follicle	Absent			Expression is not present in the ovarian follicle or the corpora lutea.
TS28: germ cell of ovary	Absent

Structure	Level	Pattern	Image	Note
TS8: epiblast	Present		1C/D	Transcripts were confined to the embryonic ectoderm through its whole extent and showed no anterior or posterior preference.
TS8: extraembryonic ectoderm	Present		1C/D

Structure	Level	Pattern	Image	Note
TS11: neural ectoderm	Present		1E/F	Expression was present in the primitive ectoderm-neural plate, and it culminated in the head fold.
TS11: embryo mesoderm	Weak		1E/F
TS11: extraembryonic mesoderm	Weak		1E/F

Structure	Level	Pattern	Image	Note
TS12: head somite	Present		2A/B
TS12: trunk somite	Present		2A/B
TS12: surface ectoderm	Present	Non-Uniform	2A/B	Expression was strongest in the cranial folds.
TS12: extraembryonic ectoderm	Present		2A/B
TS12: foregut diverticulum	Present		2A/B	Expression is present in the foregut pocket.

Structure	Level	Pattern	Image	Note
TS12: head somite	Present		2C/D
TS12: heart	Present		2C/D
TS12: trunk somite	Present		2C/D
TS12: surface ectoderm	Present		2C/D	Expression was strongest in the cranial folds.
TS12: extraembryonic ectoderm	Present		2C/D

Structure	Level	Pattern	Image	Note
TS15: embryo mesenchyme	Present		3B	Expression is present in the lateral and splanchnic mesoderm.
TS15: future brain	Present		3B	Expression is present in the cranial and dorsal neural tube.
TS15: neural tube	Present		3B	Expression is present in the cranial and dorsal neural tube.
TS15: surface ectoderm	Present		3B
TS15: neural tube lateral wall	Present	Regionally restricted	3B	Expression is present in the alar plate.
TS15: nephrogenic cord	Present		3B

Structure	Level	Pattern	Image	Note
TS15: forelimb bud ectoderm	Present	Graded	3E	Expression increased in the posterior and proximal direction towards a position close to the body wall.
TS15: neural tube lateral wall	Present	Regionally restricted	3E	Expression is present in the alar plate.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud ectoderm	Present	Graded	3F	Expression increased in the posterior and proximal direction towards a position close to the body wall.
TS15: neural tube lateral wall	Present	Regionally restricted	3F	Expression is present in the alar plate.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud ectoderm	Present	Graded	3G	Expression increased in the posterior and proximal direction towards a position close to the body wall.
TS15: neural tube lateral wall	Present	Regionally restricted	3G	Expression is present in the alar plate.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud ectoderm	Present	Graded	4B	Expression increased in the posterior and proximal direction towards a position close to the body wall.
TS17: neural tube lateral wall	Present	Regionally restricted	4B	Expression is present in the alar plate.
TS17: mesonephros	Present		4B
TS17: surface ectoderm	Present		4B

Structure	Level	Pattern	Image	Note
TS19: future arm mesenchyme	Present		4E	Expression is present in the mesenchymal aggregates corresponding to the future bones of the limb.
TS19: handplate mesenchyme	Present		4E	Expression is present in the mesenchymal aggregates corresponding to the future bones of the limb.
TS19: future arm ectoderm	Present		4E	Expression is present as a continuous contour.
TS19: handplate ectoderm	Present		4E	Expression is present as a continuous contour.
TS19: surface ectoderm	Present		4E
TS19: vertebral cartilage condensation	Present		4E
TS19: esophagus	Present		4E

Structure	Level	Pattern	Image	Note
TS20: femur pre-cartilage condensation	Present		5B	Expression is present in the chondrification centers.
TS20: hindlimb epithelium	Present		5B	Expression is present in the ectoderm.

Structure	Level	Pattern	Image	Note
TS22: forelimb phalanx cartilage condensation	Present		5E	Expression is present in the body of phalanges and carpals.
TS22: metacarpus cartilage condensation	Present		5E	Expression is present in the body of phalanges and carpals.
TS22: radius cartilage condensation	Present		5E	Expression is present in the periphery of the developing radius.
TS22: forelimb skin	Present	Non-Uniform	5E	Expression is present in the surface ectoderm of the limb; it is strongest in the interdigital web.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex	Present		6B	Expression is present in cerebral ganglia and in elements of the developing cortex.
TS20: spinal cord	Present		6B
TS20: brain	Present	Regionally restricted	6B	Expression is present in the ependymal layer.
TS20: choroid plexus	Present		6B	Expression is present in the choroid plexus.
TS20: pharynx epithelium	Present		6B
TS20: face	Absent		6B
TS20: tongue	Absent		6B
TS20: trunk mesenchyme	Absent		6B
TS20: trigeminal ganglion	Present		6B
TS20: vertebral cartilage condensation	Present		6B
TS20: intestine mesenchyme	Present	Regionally restricted	6B	Expression is present in the mesodermal wall.
TS20: lung epithelium	Present	Regionally restricted	6B	Expression is present in the alveoli.
TS20: genital tubercle	Strong	Regionally restricted	6B	Expression is present in the epithelium of the genital eminence.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present		6E	Expression is present in cerebral ganglia and in elements of the developing cortex.
TS22: spinal cord	Present		6E
TS22: brain	Present	Regionally restricted	6E	Expression is present in the ependymal layer.
TS22: choroid plexus	Present		6E	Expression is present in the choroid plexus.
TS22: pharynx epithelium	Present		6E
TS22: face	Absent		6E
TS22: tongue	Absent		6E
TS22: trunk mesenchyme	Absent		6E
TS22: sternum	Present		6E
TS22: tooth epithelium	Present		6E
TS22: vertebral cartilage condensation	Present	Regionally restricted	6E	Expression is present in the periphery.
TS22: intestine mesenchyme	Present	Regionally restricted	6E	Expression is present in the mesoderm.
TS22: lung epithelium	Present	Regionally restricted	6E	Expression is present in the alveoli.
TS22: genital tubercle	Strong	Regionally restricted	6E	Expression is present in the epithelium of the genital eminence.

Structure	Level	Pattern	Image	Note
TS17: otocyst	Present		7B	Expression is present in the otic vesicle.
TS17: pharynx epithelium	Present		7B
TS17: 1st branchial arch maxillary component	Present		7B
TS17: 1st branchial arch mandibular component	Present		7B
TS17: pharynx	Present	Regionally restricted	7B	Expression is present in the hyoid arch.
TS17: branchial arch	Present	Graded	7B	Expression is graded, increasing from the base of the arch towards its distal tip.

Structure	Level	Pattern	Image	Note
TS19: pharynx epithelium	Present		7E
TS19: hyoid cartilage primordium	Present		7E
TS19: mandibular process	Present		7E
TS19: maxillary process	Present		7E

Structure	Level	Pattern	Image	Note
TS20: face	Absent		7G
TS20: mandibular process	Present		7G
TS20: tongue	Absent		7G
TS20: trunk mesenchyme	Absent		7G
TS20: cranium	Present	Regionally restricted	7G	Expression is present in the frontal bone.
TS20: maxilla	Present		7G
TS20: forebrain	Present	Regionally restricted	7G	Expression is present in certain areas of the fore and hindbrain.
TS20: hindbrain	Present	Regionally restricted	7G	Expression is present in certain areas of the fore and hindbrain.
TS20: axial skeleton	Present	Regionally restricted	7G	Expression is present in the scapula.
TS20: skin	Present		7G	Expression is present in the surface ectoderm.

Structure	Level	Pattern	Image	Note
TS22: cochlear duct	Present		7J
TS22: utricle	Present	Regionally restricted	7J	Expression is present in the semicircular ducts.
TS22: face	Absent		7J
TS22: tongue	Absent		7J
TS22: trunk mesenchyme	Absent		7J
TS22: mandible	Present		7J	Expression is present in the mandibula.
TS22: frontal bone primordium	Present		7J
TS22: maxilla	Present		7J
TS22: forebrain	Present	Regionally restricted	7J	Expression is present in certain areas of the fore and hindbrain.
TS22: hindbrain	Present	Regionally restricted	7J	Expression is present in certain areas of the fore and hindbrain.
TS22: ossicle	Present		7J
TS22: rib cartilage condensation	Present		7J
TS22: skin	Present		7J	Expression is present in the surface ectoderm.

Structure	Level	Pattern	Image	Note
TS20: spinal cord ventricular layer	Present	Regionally restricted	8B	Expression is present in the ependymal layer.
TS20: esophagus	Present		8B
TS20: rib pre-cartilage condensation	Present		8B
TS20: vertebral cartilage condensation	Present		8B

Structure	Level	Pattern	Image	Note
TS22: spinal cord ventricular layer	Present	Regionally restricted	8E	Expression is present in the ependymal layer.
TS22: esophagus	Present		8E
TS22: lung epithelium	Present	Regionally restricted	8E	Expression is present in the alveoli.

Structure	Level	Pattern	Image	Note
TS22: spinal cord ventricular layer	Present	Regionally restricted	8H	Expression is present in the ependymal layer.
TS22: hindgut	Present	Regionally restricted	8H	Expression is present in the cloaca.
TS22: stomach epithelium	Present	Regionally restricted	8H
TS22: genital tubercle	Strong	Regionally restricted	8H	Expression is present in the epithelium of the genital eminence.
TS22: liver	Present		8H
TS22: pancreas	Present		8H

Structure	Level	Pattern	Image	Note
TS20: primitive collecting duct	Present	Regionally restricted	9B	Expression is present in the epithelium of the ureteric bud and its branches.

Structure	Level	Pattern	Image	Note
TS22: collecting duct	Present	Regionally restricted	9E	Expression is present in the epithelium of the collecting tubules.

Structure	Level	Pattern	Image	Note
TS22: epidermis	Present		10B	Expression is present in the epidermis and its derivatives, the epithelial root sheath and bulb.