Gene Expression Data
Assay Details
Assay
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Reference:
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J:34073
Adams RH, et al., A novel class of murine semaphorins with homology to thrombospondin is differentially expressed during early embryogenesis. Mech Dev. 1996 Jun;57(1):33-45
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3044159
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Gene symbol:
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Sema3a
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Gene name:
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sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A
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Probe:
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SemD cDNA3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
8A:
embryonic day 10.0
(more )
Specimen
8A:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 10.0
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Age Note:
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Age normalized so that noon of plug day = E0.5. Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
8B:
embryonic day 10.0
(more )
Specimen
8B:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 10.0
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Age Note:
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Age normalized so that noon of plug day = E0.5. Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
8C:
embryonic day 10.0
(more )
Specimen
8C:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 10.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
8D:
embryonic day 10.0
(more )
Note: Vibratome section.
Specimen
8D:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 10.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Vibratome section.
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Structure |
Level |
Pattern |
Image |
Note |
TS16: neural tube |
Present |
Regionally restricted |
8D
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Expression is in the dorsal-most portion of the neural tube.
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Specimen
8H:
embryonic day 12.5
(more )
Specimen
8H:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS20: tail sclerotome |
Present |
Regionally restricted |
8H
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Expression is in the posterior half.
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TS20: head |
Strong |
Regionally restricted |
8H
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Expression is in the branchial arches.
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Specimen
8K:
embryonic day 12.5
(more )
Specimen
8K:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
8L:
embryonic day 12.5
(more )
Specimen
8L:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
Not Shown:
embryonic day 15.5
(more )
Specimen
Not Shown:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS23: limb |
Weak |
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