Gene Expression Data
Assay Details
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Assay
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Reference:
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J:89228
Chen MH, et al., Palmitoylation is required for the production of a soluble multimeric Hedgehog protein complex and long-range signaling in vertebrates. Genes Dev. 2004 Mar 15;18(6):641-59
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3053290
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Gene symbol:
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Ptch1
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Gene name:
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patched 1
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Probe:
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Ptch1 probe5
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2H:
embryonic day 10.5
(more  )
Specimen
2H:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2I:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * FVB/N * various
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Mutant Allele(s):
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Hhattm1Ptch/Hhattm1Ptch
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: embryo |
Weak |
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2I
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Expression is greatly reduced in this mutant compared to wild-type embryos.
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Specimen
2J:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * FVB/N * various
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Mutant Allele(s):
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Shhtm1Ptch/Shhtm1Ptch
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
4I:
embryonic day 10.5
(more )
Specimen
4I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: limb |
Present |
Regionally restricted |
4I
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Specimen
4P:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * FVB/N * various
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Mutant Allele(s):
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Hhattm1Ptch/Hhattm1Ptch
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: limb |
Weak |
Regionally restricted |
4P
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Expression in this mutant is greatly reduced.
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Analysis Tools