Gene Expression Data
Assay Details
Assay
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Reference:
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J:57761
Singer JD, et al., Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells. Genes Dev. 1999 Sep 15;13(18):2375-87
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3054524
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Gene symbol:
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Krt8
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Gene name:
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keratin 8
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Antibody:
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TROMA 1
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
5D +/+:
embryonic day 7.5
(more )
Specimen
5D +/+:
(close )
Genetic Background:
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involves: 129S4/SvJaeSor * C57BL/6J
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Age:
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embryonic day 7.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5D -/-:
(close )
Genetic Background:
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involves: 129S4/SvJaeSor * C57BL/6J
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Mutant Allele(s):
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Cul3tm1Jro/Cul3tm1Jro
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Age:
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embryonic day 7.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS11: trophectoderm |
Present |
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5D Cul3-/-
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The authors state that staining is in the abnormally developed trophectoderm and that trophoblast giant cells were more sparsely represented than in wild-type embryos.
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