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Gene Expression Data
Assay Details
Assay
Reference: J:21125 Lee CH, et al., Normal onset, but prolonged expression, of Sry gene in the B6.YDOM sex-reversed mouse gonad. Dev Biol. 1994 Oct;165(2):442-52
Assay type: RT-PCR
MGI Accession ID: MGI:3497946
Gene symbol: Cyp17a1
Gene name: cytochrome P450, family 17, subfamily a, polypeptide 1
Probe: 17-HA-pA,-pB
Probe preparation: DNA
Visualized with: Ethidium bromide
Assay notes: Analyses were performed in C57BL/6J mice which contained the Y chromosome of M. m. domesticus. A large number of gonads were examined and a summary of RT-PCR analyses in B6.YDom is shown as a percentage of positive results in Figure 4.
Results
Image: 4d ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 396 bp Amount Genetic BackgroundMutant Allele(s)Sex Note
S Mol. Wt. Marker Lane
10,+ E10.5 (a) TS17: gonad primordium Absent Not Specified; total RNA involves: C57BL/6J * M. m. domesticus Not Specified Morphologically undifferentiated gonad.
10,- Control
11,+ E11.5 (a) TS19: gonad Absent Not Specified; total RNA involves: C57BL/6J * M. m. domesticus Not Specified Morphologically undifferentiated gonad.
11,- Control
12,+ E12.5 (a) TS20: gonad Absent Not Specified; total RNA involves: C57BL/6J * M. m. domesticus Not Specified Morphologically undifferentiated gonad.
12,- Control
S Mol. Wt. Marker Lane
13,+ E13.5 (a) TS21: gonad Absent Not Specified; total RNA involves: C57BL/6J * M. m. domesticus Not Specified Ovary (sex-reversed).
13,- Control
14,+ E14.5 (a) TS22: gonad Absent Not Specified; total RNA involves: C57BL/6J * M. m. domesticus Not Specified Ovary (sex-reversed).
14,- Control
15,+ E15.5 (a) TS23: gonad Absent Not Specified; total RNA involves: C57BL/6J * M. m. domesticus Not Specified Ovary (sex-reversed).
15,- Control
16,+ E16.5 (a) TS24: gonad Absent Not Specified; total RNA involves: C57BL/6J * M. m. domesticus Not Specified Ovary (sex-reversed).
16,- Control
18,+ E18.5 (a) TS26: gonad Absent Not Specified; total RNA involves: C57BL/6J * M. m. domesticus Not Specified Ovary (sex-reversed).
18,- Control
S Mol. Wt. Marker Lane
13,+ E13.5 (a) TS21: gonad Absent Not Specified; total RNA involves: C57BL/6J * M. m. domesticus Not Specified Ovotestis.
13,- Control
14,+ E14.5 (a) TS22: gonad Present Not Specified; total RNA involves: C57BL/6J * M. m. domesticus Not Specified Ovotestis.
14,- Control
15,+ E15.5 (a) TS23: gonad Weak Not Specified; total RNA involves: C57BL/6J * M. m. domesticus Not Specified Ovotestis.
15,- Control
16,+ E16.5 (a) TS24: gonad Weak Not Specified; total RNA involves: C57BL/6J * M. m. domesticus Not Specified Ovotestis.
16,- Control
18,+ E18.5 (a) TS26: gonad Weak Not Specified; total RNA involves: C57BL/6J * M. m. domesticus Not Specified Ovotestis.
18,- Control
Notes:
(a) Age normalized so that noon of plug day = E0.5.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
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