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Gene Expression Data
Assay Details
Assay
Reference: J:91257 Gray PA, et al., Mouse Brain Organization Revealed Through Direct Genome-Scale TF Expression Analysis. Science. 2004 Dec 24;306(5705):2255-2257
Assay type: RNA in situ
MGI Accession ID: MGI:3507577
Gene symbol: Pou3f1
Gene name: POU domain, class 3, transcription factor 1
Probe: MTF#217
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: Additional electronic submission not included in Supplemental Table 4 of the publication. Results obtained using this probe were the same as probe MTF#208 (MGI:3506072). Authors did not specify which probe was used when staining the specimen in Fig. S5; it is possible MTF#208 (MGI:3506072) was used.
Results
Specimen 1: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: head Present MTF# E 217 Image Expression was detected in neural and non-neural tissue.
TS21: central nervous system Present Single cells MTF# E 217 Image Expression was detected in some cells.
TS21: cerebral cortex Present Single cells MTF# E 217 Image Expression was detected in some cells.
TS21: corpus striatum Present Single cells MTF# E 217 Image Expression was detected in some cells.
TS21: thalamus Present Single cells MTF# E 217 Image Expression was detected in some cells.
TS21: hypothalamus Present Single cells MTF# E 217 Image Expression was detected in some cells.
TS21: midbrain Present Single cells MTF# E 217 Image Expression was detected in some cells.
TS21: hindbrain Present Single cells MTF# E 217 Image Expression was detected in some cells.
TS21: spinal cord Absent MTF# E 217 Image
TS21: cranial ganglion Absent MTF# E 217 Image
TS21: dorsal root ganglion Absent MTF# E 217 Image
TS21: brain ventricular layer Absent MTF# E 217 Image
TS21: retina Absent MTF# E 217 Image
TS21: ear Absent MTF# E 217 Image The tissue was inner ear and ear structures.
TS21: olfactory lobe Absent MTF# E 217 Image The tissue was olfactory epithelium and/or bulb.
Specimen 2: postnatal newborn (more )
Structure Level Pattern Image Note
TS27: head Present Regionally restricted MTF# P 217 Image Expression was neural specific.
TS27: central nervous system Present Single cells MTF# P 217 Image Expression was detected in some cells.
TS27: cerebral cortex Present Single cells MTF# P 217 Image Expression was detected in some cells.
TS27: corpus striatum Absent MTF# P 217 Image
TS27: thalamus Absent MTF# P 217 Image
TS27: hypothalamus Absent MTF# P 217 Image
TS27: midbrain Absent MTF# P 217 Image
TS27: cerebellum Absent MTF# P 217 Image
TS27: hindbrain Present Single cells MTF# P 217 Image Expression was detected in some cells.
TS27: spinal cord Present Single cells MTF# P 217 Image Expression was detected in some cells.
TS27: cranial ganglion Absent MTF# P 217 Image
TS27: dorsal root ganglion Absent MTF# P 217 Image
TS27: brain ventricular layer Absent MTF# P 217 Image
TS27: retina Present S5_Pou3f1 , MTF# P 217 Image Based on author communication, information in Fig. S5 supersedes the supplemental table S4.
TS27: ear Absent MTF# P 217 Image The tissue was inner ear and ear structures.
TS27: main olfactory bulb Absent MTF# P 217 Image The tissue was olfactory epithelium and/or bulb.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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10/22/2024
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