Gene Expression Data
Assay Details
Assay
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Reference:
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J:67054
Laurikkala J, et al., TNF signaling via the ligand-receptor pair ectodysplasin and edar controls the function of epithelial signaling centers and is regulated by Wnt and activin during tooth organogenesis. Dev Biol. 2001 Jan 15;229(2):443-55
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3513989
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Gene symbol:
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Eda
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Gene name:
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ectodysplasin-A
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Probe:
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Ta probe
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Probe preparation:
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labelled with S35 RNA
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Results
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Specimen
1A:
embryonic day 10.5
(more )
Specimen
1A:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 10.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS17: 1st branchial arch mandibular component |
Present |
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1A
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Prior to morphological tooth formation, expression throughout the simple oral epithelium covering the mandibular arch.
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TS17: oral epithelium |
Present |
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1A
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Prior to morphological tooth formation, expression throughout the simple oral epithelium covering the mandibular arch.
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Specimen
1C:
embryonic day 11.5
(more )
Specimen
1C:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS19: mandibular process |
Present |
Regionally restricted |
1C
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During initiation of tooth development, expression is reduced in the thickened presumptive dental epithelium.
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TS19: oral epithelium |
Present |
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1C
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Authors state that expression continues in oral epithelium
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Specimen
1E:
embryonic day 12.5
(more )
Specimen
1E:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 12.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
1G:
embryonic day 13.5
(more )
Specimen
1G:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS21: tooth epithelium |
Strong |
Regionally restricted |
1G
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At bud stage, intense expression in basal cells of the fully developed bud (outer epithelium of tooth germ), except for the forming signaling center at the tip, corresponding to the enamel knot.
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TS21: enamel knot |
Absent |
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1G
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TS21: oral epithelium |
Present |
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1G
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Specimen
1I:
embryonic day 14.5
(more )
Specimen
1I:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
1K:
embryonic day 17.5
(more )
Specimen
1K:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
1M:
postnatal newborn
(more )
Specimen
1M:
(close )
Genetic Background:
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NMRI
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5A:
embryonic day 11.5
(more )
Specimen
5A:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5B:
(close )
Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Lef1tm1Rug/Lef1tm1Rug
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5C:
embryonic day 13.5
(more )
Specimen
5C:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS21: tooth epithelium |
Present |
Regionally restricted |
5C
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Expression in outer epithelium of tooth bud.
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Specimen
5D:
(close )
Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Lef1tm1Rug/Lef1tm1Rug
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS21: tooth epithelium |
Weak |
Regionally restricted |
5D
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Expression significantly downregulated in dental epithelium and oral ectoderm flanking the tooth buds. No expression on lingual aspect of tooth buds; variable weak expression on buccal side.
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Specimen
5E:
embryonic day 14.5
(more )
Specimen
5E:
(close )
Genetic Background:
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NMRI
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5F:
(close )
Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Lef1tm1Rug/Lef1tm1Rug
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS22: tooth epithelium |
Weak |
Regionally restricted |
5F
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Expression on buccal but not lingual aspects of tooth germs.
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