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Gene Expression Data
Assay Details
Assay
Reference: J:23597 Cao QP, et al., Expression of the mouse testis-determining gene Sry in male preimplantation embryos. Mol Reprod Dev. 1995 Feb;40(2):196-204
Assay type: RT-PCR
MGI Accession ID: MGI:3524746
Gene symbol: Zfy1
Gene name: zinc finger protein 1, Y-linked
Probe: Zfy1-pE, Zfy1-pF
Probe preparation: Antisense labelled with P32 RNA
Assay notes: This is an RT/PCR/RNase assay: total RNA was reverse transcribed and amplified by PCR. The amplified fragments were detected by their ability to protect a riboprobe from RNase digestion. The probe used is PBSZfy1 (MGI:25409).
Results
Image: 2A ZFY ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 310 b Amount Genetic BackgroundMutant Allele(s)Sex Note
d Lane not used in this assay
P Lane not used in this assay
1 Lane not used in this assay
2 Lane not used in this assay
3 Lane not used in this assay
4 Lane not used in this assay
Lane 7 Mol. Wt. Marker Lane
5 E4.0 (a) TS5: blastocyst-stage conceptus Absent Not Specified; total RNA involves: C57BL/6 * CF-1 * SJL Not Specified Late blastocyst stage, 1.1 embryo equivalent.
6 E4.0 (a) TS5: blastocyst-stage conceptus Absent Not Specified; total RNA involves: C57BL/6 * CF-1 * SJL Not Specified Late blastocyst stage, 1.1 embryo equivalent.
7 Control
8 Control
d Control
P Control
Notes:
(a) Age of embryo at noon of plug day not specified in reference.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
06/12/2024
MGI 6.13
The Jackson Laboratory