Probe:	Plxnb1 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	The authors state that identical patterns were observed with the following probe: Plxnb1 cDNA2 (MGI:3531011).

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex mantle layer	Strong
TS22: cerebral cortex ventricular layer	Strong
TS22: intestine	Strong
TS22: lung epithelium	Strong
TS22: metanephros	Strong
TS22: midbrain	Strong
TS22: olfactory epithelium	Strong
TS22: olfactory lobe	Strong
TS22: oral epithelium	Strong
TS22: retina	Strong
TS22: tooth	Strong
TS22: diencephalon	Strong	Regionally restricted		Expression is in the optic recess of the diencephalon.
TS22: cerebral cortex marginal layer	Moderate
TS22: choroid plexus	Moderate
TS22: corpus striatum	Moderate
TS22: dorsal root ganglion	Moderate
TS22: remnant of Rathke's pouch	Moderate
TS22: trigeminal ganglion	Moderate
TS22: epidermis	Weak
TS22: lung mesenchyme	Weak
TS22: spinal cord	Weak
TS22: cochlea	Absent
TS22: endocardial tissue	Absent
TS22: pre-cartilage condensation	Absent
TS22: skeletal muscle	Absent

Structure	Level	Pattern	Image	Note
TS22: lung epithelium	Strong		1_Plxnb1
TS22: metanephros	Strong		1_Plxnb1
TS22: olfactory epithelium	Strong		1_Plxnb1
TS22: oral epithelium	Strong		1_Plxnb1
TS22: tooth	Strong		1_Plxnb1
TS22: enteric nervous system	Strong	Regionally restricted	1_Plxnb1	Expression is in the lamina propria, muscularis externa and the enteric nervous plexus.
TS22: intestine	Strong	Regionally restricted	1_Plxnb1	Expression is in the lamina propria, muscularis externa and the enteric nervous plexus.
TS22: lens anterior epithelium	Present		1_Plxnb1
TS22: neural retinal epithelium	Present		1_Plxnb1
TS22: olfactory lobe	Present		1_Plxnb1
TS22: optic stalk	Present		1_Plxnb1
TS22: remnant of Rathke's pouch	Present		1_Plxnb1
TS22: diencephalon	Present	Regionally restricted	1_Plxnb1	Expression is in the optic recess of the diencephalon.
TS22: epidermis	Weak		1_Plxnb1
TS22: lung mesenchyme	Weak		1_Plxnb1

Structure	Level	Pattern	Image	Note
TS22: maturing glomerular tuft	Present	Regionally restricted	2A	Expression is in the podocytes.
TS22: renal cortex	Present	Regionally restricted	2A	Expression is in undifferentiated mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: maturing glomerular tuft	Present	Regionally restricted	2B	Expression is in the podocytes.
TS22: renal cortex	Present	Regionally restricted	2B	Expression is in undifferentiated mesenchyme.

Structure	Level	Pattern	Image	Note
TS24: renal cortex	Present	Regionally restricted	2E	Expression is in undifferentiated mesenchyme.

Structure	Level	Pattern	Image	Note
TS24: renal cortex	Present	Regionally restricted	2F	Expression is in undifferentiated mesenchyme.

Structure	Level	Pattern	Image	Note
TS26: renal cortex	Present	Regionally restricted	2I	Expression is in the very outermost cortex where immature glomeruli and mesenchyme are still present.

Structure	Level	Pattern	Image	Note
TS26: renal cortex	Present	Regionally restricted	2J	Expression is in the very outermost cortex where immature glomeruli and mesenchyme are still present.

Structure	Level	Pattern	Image	Note
TS22: enamel knot	Present		3B
TS22: oral epithelium	Present		3B
TS22: inner enamel epithelium	Present	Regionally restricted	3B	Expression is in the basal layer.
TS22: outer enamel epithelium	Present	Regionally restricted	3B	Expression is in the basal layer.

Structure	Level	Pattern	Image	Note
TS23: enamel knot	Present		3E
TS23: oral epithelium	Present		3E
TS23: inner enamel epithelium	Present	Regionally restricted	3E	Expression is in the basal layer.
TS23: outer enamel epithelium	Present	Regionally restricted	3E	Expression is in the basal layer.

Structure	Level	Pattern	Image	Note
TS24: enamel knot	Present		3H
TS24: oral epithelium	Present		3H
TS24: inner enamel epithelium	Present	Regionally restricted	3H	Expression is in the basal layer.
TS24: outer enamel epithelium	Present	Regionally restricted	3H	Expression is in the basal layer.

Structure	Level	Pattern	Image	Note
TS24: seminiferous cord	Present		4B

Structure	Level	Pattern	Image	Note
TS27: testis	Present	Regionally restricted	4E	Expression is in the seminiferous tubules.

Structure	Level	Pattern	Image	Note
TS26: seminiferous cord	Present