Gene Expression Data
Assay Details
Assay
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Reference:
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J:55464
Rivolta MN, et al., GATA3 is downregulated during hair cell differentiation in the mouse cochlea. J Neurocytol. 1998 Sep;27(9):637-47
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3575418
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Gene symbol:
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Gata3
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Gene name:
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GATA binding protein 3
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Antibody:
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GATA-3 antibody
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Detection system:
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Secondary antibody coupled to rhodamine
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Assay notes:
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The fixation was in methanol/acetone.
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Results
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Specimen
1B:
embryonic day 14.5
(more )
Specimen
1B:
(close )
Genetic Background:
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C57BL/6
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Age:
|
embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
1C:
embryonic day 14.5
(more )
Specimen
1C:
(close )
Genetic Background:
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C57BL/6
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Age:
|
embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
2B:
embryonic day 18.5
(more )
Specimen
2B:
(close )
Genetic Background:
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C57BL/6
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Age:
|
embryonic day 18.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS26: organ of Corti |
Present |
Regionally restricted |
2B
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This view is of the epithelium of the apical turn.
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Specimen
2D:
embryonic day 18.5
(more )
Specimen
2D:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
embryonic day 18.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
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Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
Other - See Notes
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Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: organ of Corti |
Weak |
Regionally restricted |
2D
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This view is of the basal turn. Labeling is in the epithelium and labeling in the hair cells has decreased.
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Specimen
2E:
embryonic day 18.5
(more )
Specimen
2E:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
embryonic day 18.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: organ of Corti |
Weak |
Regionally restricted |
2E
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This view is of the basal turn. Labeling is in the epithelium and labeling in the hair cells has decreased.
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Specimen
3A:
embryonic day 18.5
(more )
Specimen
3A:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
embryonic day 18.5
|
Age Note:
|
Age normalized so that noon of plug day = E0.5.
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Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
Other - See Notes
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Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: organ of Corti |
Present |
Regionally restricted |
3A
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This view is or the basal turn. The outer hair cells show little label, but the inner hair cells show positive labeling. There is strong labeling in phalangeal cells, Deiter's cells, pillar cells and Hensen's cells.
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Specimen
4B:
postnatal day 0
(more )
Specimen
4B:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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Other - See Notes
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Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: organ of Corti |
Present |
Single cells |
4B
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In the basal turn, labeling intensity has decreased in all cells. This includes supporting cells in the organ of Corti, from the base to the apex of the cochlea. In the apical turn, hair cells were not labeled, while Deiter's cells remained positive.
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Specimen
5A:
postnatal day 14
(more )
Specimen
5A:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 14
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS28: organ of Corti |
Absent |
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5A
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Expression is absent in the entire epithelium.
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Specimen
7B:
embryonic day 16.5
(more )
Specimen
7B:
(close )
Genetic Background:
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C57BL/6
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Age:
|
embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS24: cochlear duct epithelium |
Present |
Single cells |
7B
,
7A
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The spiral ganglion cells are positively labeled. There is a decrease in the labeling of the hair cells in the basal turn that is described in the text.
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Specimen
8B:
embryonic day 16.5
(more )
Specimen
8B:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
Not shown:
postnatal day 6
(more )
Specimen
Not shown:
(close )
Genetic Background:
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C57BL/6
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Age:
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postnatal day 6
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: organ of Corti |
Weak |
Single cells |
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Only scattered cells remain positive.
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Specimen
6 E15:
embryonic day 15.5
(more )
Specimen
6 E15:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: cochlea |
Present |
Regionally restricted |
6
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Expression detected in hair cells of the cochlea. Data is a graphical representation of multiple sections presented as a % of hair cells expressing Gata3. 100% expressed Gata3 at E15.5.
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Specimen
6 E14:
embryonic day 14.5
(more )
Specimen
6 E14:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
Other - See Notes
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Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: cochlea |
Present |
Regionally restricted |
6
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Expression detected in hair cells of the cochlea. Data is a graphical representation of multiple sections presented as a % of hair cells expressing Gata3. 100% expressed Gata3 at E14.5.
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Specimen
6 E16:
embryonic day 16.5
(more )
Specimen
6 E16:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
Other - See Notes
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Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: cochlea |
Present |
Regionally restricted |
6
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Expression detected in hair cells of the cochlea. Data is a graphical representation of multiple sections presented as a % of hair cells expressing Gata3. 80% expressed Gata3 at E16.5.
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Specimen
6 E 18:
embryonic day 18.5
(more )
Specimen
6 E 18:
(close )
Genetic Background:
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C57BL/6
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Age:
|
embryonic day 18.5
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Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: cochlea |
Weak |
Regionally restricted |
6
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Expression detected in hair cells of the cochlea. Data is a graphical representation of multiple sections presented as a % of hair cells expressing Gata3. 30% expressed Gata3 at E18.5.
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Specimen
6 P0:
postnatal day 0
(more )
Specimen
6 P0:
(close )
Genetic Background:
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C57BL/6
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Age:
|
postnatal day 0
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Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: cochlea |
Weak |
Regionally restricted |
6
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Expression detected in hair cells of the cochlea. Data is a graphical representation of multiple sections presented as a % of hair cells expressing Gata3. 15% expressed Gata3 at P0.
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Specimen
6 P14:
postnatal day 14.5
(more )
Specimen
6 P14:
(close )
Genetic Background:
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C57BL/6
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Age:
|
postnatal day 14.5
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Sex:
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Not Specified
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Type:
|
section
|
Fixation:
|
Other - See Notes
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Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: cochlea |
Absent |
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6
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Data is a graphical representation of multiple sections presented as a % of hair cells expressing Gata3. 0% expressed Gata3 at P14.
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