Probe:	Dag1 cDNA1
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS15: embryo	Present		1B
TS15: heart	Strong		1B
TS15: surface ectoderm	Strong		1B

Structure	Level	Pattern	Image	Note
TS20: embryo	Moderate		1D	Ubiquitous expression.
TS20: 3rd ventricle	Strong	Regionally restricted	1D	Expression is in the ependymal cells lining the brain and spinal cord.
TS20: 4th ventricle	Strong	Regionally restricted	1D	Expression is in the ependymal cells lining the brain and spinal cord.
TS20: lateral ventricle	Strong	Regionally restricted	1D	Expression is in the ependymal cells lining the brain and spinal cord.
TS20: spinal cord ventricular layer	Strong	Regionally restricted	1D	Expression is in the ependymal cells lining the brain and spinal cord.
TS20: heart	Strong		1D
TS20: left lung lobar bronchus	Strong		1D
TS20: right lung lobar bronchus	Strong		1D
TS20: skeletal muscle	Strong		1D

Structure	Level	Pattern	Image	Note
TS23: heart	Very strong		1E
TS23: skeletal muscle	Very strong		1E

Structure	Level	Pattern	Image	Note
TS20: cardiac muscle tissue	Strong
TS20: skeletal muscle	Strong
TS20: lung	Strong	Regionally restricted		Expression is in bronchi wall smooth muscle.
TS20: 3rd ventricle	Strong	Regionally restricted		Expression is in the ependymal cells.
TS20: 4th ventricle	Strong	Regionally restricted		Expression is in the ependymal cells.
TS20: lateral ventricle	Strong	Regionally restricted		Expression is in the ependymal cells.
TS20: metanephros	Moderate
TS20: central nervous system ganglion	Weak
TS20: pituitary gland	Weak
TS20: vibrissa	Weak
TS20: mandibular process	Weak			Expression is in teeth.
TS20: maxillary process	Weak			Expression is in teeth.
TS20: limb digit	Trace	Regionally restricted		Expression is in tendon primordia.
TS20: cartilage	Trace	Regionally restricted		Expression is in ossifying cartilages.

Structure	Level	Pattern	Image	Note
TS23: cardiac muscle tissue	Strong
TS23: skeletal muscle	Strong
TS23: lung	Strong	Regionally restricted		Expression is in bronchi wall smooth muscle.
TS23: 3rd ventricle	Strong	Regionally restricted		Expression is in the ependymal cells.
TS23: 4th ventricle	Strong	Regionally restricted		Expression is in the ependymal cells.
TS23: lateral ventricle	Strong	Regionally restricted		Expression is in the ependymal cells.
TS23: metanephros	Moderate
TS23: central nervous system ganglion	Weak
TS23: pituitary gland	Weak
TS23: tooth	Weak
TS23: vibrissa	Weak
TS23: limb digit	Trace	Regionally restricted		Expression is in tendon primordia.
TS23: cartilage	Trace	Regionally restricted		Expression is in ossifying cartilages.

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Weak		2A
TS20: spinal cord ventricular layer	Present		2A

Structure	Level	Pattern	Image	Note
TS20: cranial ganglion	Weak		2D
TS20: pituitary gland	Weak		2D

Structure	Level	Pattern	Image	Note
TS23: 3rd ventricle	Present		2F
TS23: 4th ventricle	Present		2F
TS23: lateral ventricle	Present		2F
TS23: tooth	Absent		2F

Structure	Level	Pattern	Image	Note
TS15: embryo	Present		3B	Expression is the same as in wild-type embryos.

Structure	Level	Pattern	Image	Note
TS23: cardiac muscle tissue	Present		4A
TS23: axial musculature	Present		4A
TS23: intercostal muscle	Present		4A
TS23: tongue muscle	Present		4A
TS23: lung	Present	Regionally restricted	4A	Expression is in the smooth muscle of the bronchi.

Structure	Level	Pattern	Image	Note
TS23: cardiac muscle tissue	Present		4B
TS23: axial musculature	Present		4B
TS23: intercostal muscle	Present		4B
TS23: tongue muscle	Present		4B
TS23: lung	Present	Regionally restricted	4B	Expression is in the smooth muscle of the bronchi.