Gene Expression Data
Assay Details
Assay
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Reference:
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J:97068
Cachaco AS, et al., Integrin repertoire on myogenic cells changes during the course of primary myogenesis in the mouse. Dev Dyn. 2005 Apr;232(4):1069-78
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3578004
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Gene symbol:
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Myog
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Gene name:
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myogenin
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Antibody:
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Anti-Myogenin F5D
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Assay notes:
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The fixative was 0.2% paraformaldehyde in 0.1M phosphate buffer with 0.12mM CaCl2, 4% sucrose.
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Results
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Specimen
2H:
embryonic day 12.5
(more )
Note: Double labeled: red - Myog; green - Fn1 (assay MGI:3578021).
Specimen
2H:
(close )
Genetic Background:
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CD-1
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Myog; green - Fn1 (assay MGI:3578021).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: body wall mesenchyme |
Present |
Regionally restricted |
2H
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Expression was detected in myogenic cells in abdominal wall muscle masses.
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Specimen
3H:
embryonic day 12.5
(more )
Note: Double labeled: red - Myog; green - Fn1 (assay MGI:3578021).
Specimen
3H:
(close )
Genetic Background:
|
CD-1
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Myog; green - Fn1 (assay MGI:3578021).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: forelimb mesenchyme |
Present |
Regionally restricted |
3H
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Expression was detected in myogenic cells.
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