Probe:	Fbln2 oligo
Probe preparation:	Antisense labelled with S35 DNA
Assay notes:	The probe used in this assay was 3' labelled.

Structure	Level	Pattern	Image	Note
TS17: endocardial cushion	Strong
TS17: heart	Strong	Regionally restricted		Expression is in the epicardium.

Structure	Level	Pattern	Image	Note
TS21: cartilage	Strong		4A/B	Expression includes thyroid cartilage.
TS21: laryngeal cartilage	Strong		4A/B
TS21: mandible	Strong		4A/B
TS21: nasal septum	Strong		4A/B
TS21: femur cartilage condensation	Strong	Regionally restricted	4A/B	Expression is in the perichondrium.
TS21: vertebral cartilage condensation	Strong	Regionally restricted	4A/B	Expression is in the perichondrium.
TS21: dorsal aorta	Strong	Regionally restricted	4A/B	Expression is in smooth muscle.
TS21: trunk mesenchyme	Present	Regionally restricted	4A/B	Expression is in the outermost connective tissue that forms demarcations between individual tissues. Such as the connective tissue sheaths which cover the heart, lung, liver, and kidney.
TS21: head mesenchyme	Present	Regionally restricted	4A/B	Expression is in tissues derived from the neural crest mesenchyme.
TS21: axial skeleton	Present	Regionally restricted	4A/B	Expression is in the intervertebral discs.
TS21: blood vessel	Present	Regionally restricted	4A/B	Expression is in some endothelial cells of the liver and lung.
TS21: visceral pleura	Present		4A/B
TS21: greater sac mesothelium	Present		4A/B
TS21: metanephros	Present	Regionally restricted	4A/B	Expression is in the blood vessels and mesothelial layer of the kidney.
TS21: forebrain	Absent		4A/B
TS21: midbrain	Absent		4A/B

Structure	Level	Pattern	Image	Note
TS22: humerus cartilage condensation	Strong	Regionally restricted	5C/D	Expression is in the perichondrium absent in chondroblasts.
TS22: scapula cartilage condensation	Strong	Regionally restricted	5C/D	Expression is in the perichondrium absent in chondroblasts.

Structure	Level	Pattern	Image	Note
TS22: hindlimb phalanx cartilage condensation	Strong		5G/H

Structure	Level	Pattern	Image	Note
TS21: temporal bone petrous part	Strong		6C/D

Structure	Level	Pattern	Image	Note
TS21: laryngeal cartilage	Strong		7C/D
TS21: mandible	Strong		7C/D
TS21: nasal septum	Strong		7C/D
TS21: cartilage	Strong	Regionally restricted	7C/D	Expression is in thyroid cartilage.
TS21: nose	Strong	Regionally restricted	7C/D	Expression is in nasal cartilage.
TS21: tooth epithelium	Absent		7C/D

Structure	Level	Pattern	Image	Note
TS26: nasal capsule	Weak	Regionally restricted	8C/D
TS26: nasal septum mesenchyme	Weak	Regionally restricted	8C/D

Structure	Level	Pattern	Image	Note
TS21: vibrissa dermal component	Strong		9C/D

Structure	Level	Pattern	Image	Note
TS26: head mesenchyme	Absent		9G/H	Expression is absent surrounding the vibrissae.

Structure	Level	Pattern	Image	Note
TS23: dental papilla	Weak		11C/D

Structure	Level	Pattern	Image	Note
TS26: dental papilla	Weak		11G/H
TS26: enamel organ	Weak		11G/H

Structure	Level	Pattern	Image	Note
TS22: 4th ventricle	Present	Regionally restricted	14C/D	Expression is in the ependymal layer.
TS22: cerebellum	Present	Regionally restricted	14C/D	Expression is at the junction of cerebellar primordium and the roof of the midbrain.
TS22: midbrain roof plate	Present	Regionally restricted	14C/D	Expression is at the junction of cerebellar primordium and the roof of the midbrain.

Structure	Level	Pattern	Image	Note
TS22: spinal cord meninges	Absent		14G/H
TS22: basal columns	Present		14G/H

Structure	Level	Pattern	Image	Note
TS20: Meckel's cartilage	Present

Structure	Level	Pattern	Image	Note
TS21: Meckel's cartilage	Weak
TS21: testis	Present	Regionally restricted		Expression is in gonocytes within seminiferous tubules and in cells in the layer of peritoneal mesothelium.

Structure	Level	Pattern	Image	Note
TS22: Meckel's cartilage	Absent

Structure	Level	Pattern	Image	Note
TS23: Meckel's cartilage	Absent

Structure	Level	Pattern	Image	Note
TS26: Meckel's cartilage	Absent