Gene Expression Data
Assay Details
Assay
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Reference:
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J:45336
Gad JM, et al., The expression patterns of guidance receptors, DCC and Neogenin, are spatially and temporally distinct throughout mouse embryogenesis. Dev Biol. 1997 Dec 15;192(2):258-73
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3582395
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Gene symbol:
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Dcc
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Gene name:
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DCC netrin 1 receptor
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Probe:
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MB18
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Probe preparation:
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Antisense labelled with P33 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
1B:
embryonic day 8.5
(more )
Note: This is an oblique section.
Specimen
1B:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 8.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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This is an oblique section.
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Specimen
1E:
embryonic day 9.5
(more )
Note: This is a parasagittal section.
Specimen
1E:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 9.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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This is a parasagittal section.
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Specimen
1H:
embryonic day 11.5
(more )
Note: This is a parasagittal section.
Specimen
1H:
(close )
Genetic Background:
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ICR
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Age:
|
embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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This is a parasagittal section.
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Structure |
Level |
Pattern |
Image |
Note |
TS19: brain |
Strong |
Regionally restricted |
1H
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There is nearly ubiquitous expression in postmitotic neurons within the early central nervous system.
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TS19: future spinal cord |
Strong |
Regionally restricted |
1H
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There is strong expression in both the thoracic and tail region of the neural tube. This expression is predominantly in the dorsal regions of the neural tube where the cell bodies of interneurons and commissural neurons reside.
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TS19: central nervous system |
Present |
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1H
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No expression was deteted in tissues lying outside the developing central nervous system, nor was it present in the floor plate and neural crest.
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TS19: notochord |
Absent |
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1H
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TS19: peripheral nervous system |
Absent |
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1H
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Specimen
2B:
embryonic day 13.5
(more )
Specimen
2B:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 13.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
3:
embryonic day 13.5
(more )
Specimen
3:
(close )
Genetic Background:
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ICR
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Age:
|
embryonic day 13.5
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS21: cerebral cortex |
Strong |
Regionally restricted |
3E
,
3B
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There is strong expression in the preplate. Expression is most intense in those neurons residing at the inner and outer edge, while the centrally placed neurons express at significantly lower levels.
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Specimen
4B:
embryonic day 17.5
(more )
Note: This is a parasagittal section.
Specimen
4B:
(close )
Genetic Background:
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ICR
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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This is a parasagittal section.
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Specimen
4E:
embryonic day 18.5
(more )
Note: This is a parasagittal section.
Specimen
4E:
(close )
Genetic Background:
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ICR
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Age:
|
embryonic day 18.5
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Paraffin
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Note:
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This is a parasagittal section.
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: brain |
Present |
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4E
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Expression has been extinguished in most brain regions by this age.
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TS26: cerebral cortex |
Absent |
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4E
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TS26: midbrain |
Present |
Regionally restricted |
4E
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Expression is restricted to several structures, including the substantial nigra, the zona aserta, and the red cell nucleus.
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TS26: cerebellum |
Present |
Regionally restricted |
4E
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Expression is present in the external granular layer.
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TS26: hippocampus |
Present |
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4E
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Specimen
4H:
embryonic day 18.5
(more )
Note: This is a parasagittal section.
Specimen
4H:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
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This is a parasagittal section.
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: cerebellum |
Strong |
Regionally restricted |
4H
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Expression is present in the external granular layer.
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Specimen
5B:
embryonic day 18.5
(more )
Note: This is a coronal section taken immediately rostral to the ear.
Specimen
5B:
(close )
Genetic Background:
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ICR
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Age:
|
embryonic day 18.5
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Paraffin
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Note:
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This is a coronal section taken immediately rostral to the ear.
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: olfactory lobe |
Strong |
Regionally restricted |
5B
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Expression is restricted to the presumptive granular cell layer within the olfactory bulb.
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Specimen
5E:
embryonic day 18.5
(more )
Note: This is a coronal section taken immediately rostral to the ear.
Specimen
5E:
(close )
Genetic Background:
|
ICR
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Age:
|
embryonic day 18.5
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
This is a coronal section taken immediately rostral to the ear.
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: olfactory lobe |
Strong |
Regionally restricted |
5E
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Expression is restricted to the presumptive granular cell layer within the olfactory bulb.
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Specimen
5H:
embryonic day 18.5
(more )
Note: This is a coronal section.
Specimen
5H:
(close )
Genetic Background:
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ICR
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Age:
|
embryonic day 18.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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This is a coronal section.
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Specimen
5K:
embryonic day 18.5
(more )
Note: This is a coronal section.
Specimen
5K:
(close )
Genetic Background:
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ICR
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Age:
|
embryonic day 18.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
|
Note:
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This is a coronal section.
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: hippocampus |
Strong |
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5K
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There is strong expression in the CA1, CA2, CA3, and CA4 regions of the hippocampus.
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Specimen
6B:
embryonic day 11.5
(more )
Specimen
6B:
(close )
Genetic Background:
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ICR
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Age:
|
embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6D:
embryonic day 11.5
(more )
Specimen
6D:
(close )
Genetic Background:
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ICR
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Age:
|
embryonic day 11.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: telencephalon |
Strong |
Regionally restricted |
6D
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Expression is present in the early postmitotic neurons of the lateral eminence.
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Specimen
6F:
embryonic day 11.5
(more )
Specimen
6F:
(close )
Genetic Background:
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ICR
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Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: future spinal cord |
Strong |
Regionally restricted |
6F
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There is expression in both the thoracic and tail regions of the neural tube. This expression is predominantly in the dorsal regions, where the cell bodies of interneurons and commissural neurons reside. Lower levels were detected in the motor columns.
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TS19: neural tube ventricular layer |
Absent |
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6F
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Specimen
7B:
embryonic day 12.5
(more )
Note: This is a parasagittal section.
Specimen
7B:
(close )
Genetic Background:
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ICR
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Age:
|
embryonic day 12.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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This is a parasagittal section.
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Structure |
Level |
Pattern |
Image |
Note |
TS20: limb mesenchyme |
Present |
Regionally restricted |
7A/B
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Expression is present in mesodermal condensations around the cartilage core in the developing limb structures.
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Specimen
7D:
embryonic day 13.5
(more )
Specimen
7D:
(close )
Genetic Background:
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ICR
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Age:
|
embryonic day 13.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
7F:
embryonic day 13.5
(more )
Specimen
7F:
(close )
Genetic Background:
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ICR
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Age:
|
embryonic day 13.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Paraffin
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Specimen
7H:
embryonic day 15.5
(more )
Specimen
7H:
(close )
Genetic Background:
|
ICR
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Age:
|
embryonic day 15.5
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
|
Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS23: reproductive system |
Strong |
Regionally restricted |
7G/H
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There is strong expression at the tip of the genital tubercle within the developing urogenital region.
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Specimen
Not shown:
embryonic day 12.5
(more )
Specimen
Not shown:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: skeletal muscle |
Present |
Regionally restricted |
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Expression is present in the facial musculature, particularly around the developing palate.
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Specimen
Not shown:
embryonic day 14.5
(more )
Specimen
Not shown:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 14.5
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: limb mesenchyme |
Absent |
|
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There is no expression in mesodermal condensations around the cartilage core in the developing limb structures.
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Specimen
Not shown:
embryonic day 15.5
(more )
Specimen
Not shown:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
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Specimen
Not shown:
embryonic day 16.5
(more )
Specimen
Not shown:
(close )
Genetic Background:
|
ICR
|
Age:
|
embryonic day 16.5
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Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: superior cervical ganglion |
Present |
|
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There is no other expression in the peripheral nervous system.
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TS24: reproductive system |
Strong |
Regionally restricted |
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There is strong expression at the tip of the genital tubercle within the developing urogenital region.
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TS24: bladder |
Present |
Regionally restricted |
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There are relatively high levels of expression in the mesenchyme lying directly adjacent to the epithelium. There is no expression in the epithelial layer.
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