Sox4 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:93300 Blackshaw S, et al., Genomic analysis of mouse retinal development. PLoS Biol. 2004 Oct;2(9):E247
Assay type:	RNA in situ
MGI Accession ID:	MGI:3585488
Gene symbol:	Sox4
Gene name:	SRY (sex determining region Y)-box 4

Probe:	UI-M-BH2.1-apr-f-10-0-UI.s1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	This data is from Supplemental Table 5, Gene column, row: 18789 SRY-box containing gene 4. After cryosectioning, tissue was fixed for 10' in 4% paraformaldehyde prior to processing.

Results

Submitted Image - Sox4e12 ©

Specimen E12: embryonic day 12.0 (more

)

Structure	Level	Pattern	Image	Note
TS20: neural retinal epithelium	Weak	Regionally restricted	Submitted Image - Sox4e12	Expression was detected in the inner neuroblastic layer.

2(Continued)B Sox4 E14 ©

Submitted Image - Sox4e14 ©

Specimen E14: embryonic day 14.0 (more

)

Structure	Level	Pattern	Image	Note
TS22: neural retinal epithelium	Present	Regionally restricted	2(Continued)B Sox4 E14 , Submitted Image - Sox4e14	Expression was detected in the inner neuroblastic layer and in a small subset of cells in the outer neuroblastic layer.

2(Continued)B Sox4 E16 ©

Submitted Image - Sox4e16 ©

Specimen E16: embryonic day 16.0 (more

)

Structure	Level	Pattern	Image	Note
TS24: retina nuclear layer	Present	Regionally restricted	Submitted Image - Sox4e16 , 2(Continued)B Sox4 E16	Expression was detected in the scleral inner neuroblastic layer and a subset of cells in the outer neuroblastic layer.

2(Continued)B Sox4 E18 ©

Submitted Image - Sox4e18 ©

Specimen E18: embryonic day 18.0 (more

)

Structure	Level	Pattern	Image	Note
TS26: retina inner nuclear layer	Present	Regionally restricted	Submitted Image - Sox4e18 , 2(Continued)B Sox4 E18	Expression was detected in the scleral inner neuroblastic layer.
TS26: retina outer nuclear layer	Present	Regionally restricted	Submitted Image - Sox4e18 , 2(Continued)B Sox4 E18	Expression was detected in a subset of cells in the outer neuroblastic layer.

2(Continued)B Sox4 P0 ©

Submitted Image - Sox4p0 ©

Specimen P0/1: postnatal day 0-1 (more

)

Structure	Level	Pattern	Image	Note
TS27: retina inner nuclear layer	Present	Regionally restricted	2(Continued)B Sox4 P0 , Submitted Image - Sox4p0	Expression was detected in the scleral inner neuroblastic layer.
TS27: retina outer nuclear layer	Present	Regionally restricted	Submitted Image - Sox4p0 , 2(Continued)B Sox4 P0	Expression was detected in a subset of cells in the outer neuroblastic layer.

2(Continued)B Sox4 P2 ©

Submitted Image - Sox4p2 ©

Specimen P2/3: postnatal day 2-3 (more

)

Structure	Level	Pattern	Image	Note
TS27: retina inner nuclear layer	Present	Regionally restricted	2(Continued)B Sox4 P2 , Submitted Image - Sox4p2	Expression was detected in the scleral inner neuroblastic layer.
TS27: retina outer nuclear layer	Present	Regionally restricted	2(Continued)B Sox4 P2 , Submitted Image - Sox4p2	Expression was detected in the scleral outer neuroblastic layer.

2(Continued)B Sox4 P4 ©

Submitted Image - Sox4p4 ©

Specimen P4/5: postnatal day 4-5 (more

)

Structure	Level	Pattern	Image	Note
TS28: retina outer nuclear layer	Present	Regionally restricted	Submitted Image - Sox4p4 , 2(Continued)B Sox4 P4	Expression was detected in the vitreal outer neuroblastic layer.

2(Continued)B Sox4 P6 ©

Specimen P6/7: postnatal day 6-7 (more

)

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present		2(Continued)B Sox4 P6 , Submitted Image - Sox4p6

Specimen P8/10: postnatal day 8-10 (more

)

Structure	Level	Pattern	Image	Note
TS28: retina inner nuclear layer	Present	Regionally restricted	Submitted Image - Sox4p8	Expression was detected in a subset of cells in the inner nuclear layer.

Specimen Adult: postnatal adult (more

)

Structure	Level	Pattern	Image	Note
TS28: retina	Absent		2(Continued)B Sox4 Adult , Submitted Image - ah12ad

Specimen CNS: postnatal day 6 (more

)

Structure	Level	Pattern	Image	Note
TS28: brain subventricular zone	Present
TS28: hippocampus	Present	Regionally restricted		Expression was detected in a subset of the hippocampus.

Specimen Embryo: embryonic day 16.0 (more

)

Structure	Level	Pattern	Image	Note
TS24: central nervous system	Present

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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