Gene Expression Data
Assay Details
Assay
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Reference:
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J:102143
Yoshikawa T, et al., High-throughput screen for genes predominantly expressed in the ICM of mouse blastocysts by whole mount in situ hybridization. Gene Expr Patterns. 2006 Jan;6(2):213-24
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3607340
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Gene symbol:
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Zfp219
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Gene name:
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zinc finger protein 219
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Probe:
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NIA clone H3014D06
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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This data is reported in Table 1; it is pictured in Figure 2.
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Results
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Specimen
blastocyst:
embryonic day 3.5
(more )
Specimen
blastocyst:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 3.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS5: inner cell mass |
Strong |
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2B Zfp219
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When a less concentrated dilution of probe is used, no expression is seen.
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TS5: trophectoderm |
Present |
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2B Zfp219
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Expression levels are lower in the trophectoderm than in the inner cell mass. When a less concentrated dilution of probe is used, no expression is seen.
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