Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:102143
Yoshikawa T, et al., High-throughput screen for genes predominantly expressed in the ICM of mouse blastocysts by whole mount in situ hybridization. Gene Expr Patterns. 2006 Jan;6(2):213-24
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:3607343
|
|
Gene symbol:
|
Zfp296
|
|
Gene name:
|
zinc finger protein 296
|
|
Probe:
|
NIA clone H3086D06
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
Assay notes:
|
This data is reported in Table 1; it is pictured in Figure 2.
|
|
|
Results
|
Specimen
blastocyst:
embryonic day 3.5
(more  )
Specimen
blastocyst:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 3.5
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS5: inner cell mass |
Present |
|
2B Zfp296
|
When a less concentrated dilution of probe is used, only trace expression is seen.
|
| TS5: trophectoderm |
Present |
|
2B Zfp296
|
Expression levels are lower in the trophectoderm than in the inner cell mass. When a less concentrated dilution of probe is used, no expression is seen.
|
|
Analysis Tools
