Gene Expression Data
Assay Details
Assay
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Reference:
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J:54733
Feinstein Y, et al., F-spondin and mindin: two structurally and functionally related genes expressed in the hippocampus that promote outgrowth of embryonic hippocampal neurons. Development. 1999 Aug;126(16):3637-48
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3630267
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Gene symbol:
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Spon1
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Gene name:
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spondin 1, (f-spondin) extracellular matrix protein
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Probe:
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F-spondin cDNA1
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Probe preparation:
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Antisense RNA
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Assay notes:
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The probe was labeled with either Digoxigenin or 35S-dUTP.
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Results
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Specimen
3C:
embryonic day 15.0
(more )
Specimen
3C:
(close )
Genetic Background:
|
Not Specified
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
3D:
embryonic day 18.0
(more )
Specimen
3D:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 18.0
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
3E:
embryonic day 18.0
(more )
Specimen
3E:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 18.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: main olfactory bulb |
Present |
Regionally restricted |
3E
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Expression was detected in the pre-glomerular cells that surround the glomeruli, in the mitral cell layer and in the internal granular layer.
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Specimen
3F:
embryonic day 18.0
(more )
Specimen
3F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: cerebral cortex |
Present |
Regionally restricted |
3F
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Expression was prominent in layer V but was weaker in the other neocortical layers.
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TS26: dentate gyrus |
Present |
Regionally restricted |
3F
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Expression was detected in granule cells.
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TS26: hippocampus |
Present |
Regionally restricted |
3F
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Expression was detected in pyramidal neurons in the CA1 and CA2 regions.
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Specimen
3G:
postnatal adult
(more )
Specimen
3G:
(close )
Genetic Background:
|
Not Specified
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Age:
|
postnatal adult
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Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
|
Specimen
not shown:
embryonic day 10.0-11.0
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 10.0-11.0
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
not shown:
embryonic day 12.0
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 12.0
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Age Note:
|
Age of embryo at noon of plug day not specified in reference.
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
not shown:
embryonic day 18.0
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 18.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: diencephalon |
Strong |
Regionally restricted |
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Expression was detected in the ventral midline.
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TS26: hindbrain |
Strong |
Regionally restricted |
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Expression was detected in the ventral midline.
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TS26: midbrain |
Strong |
Regionally restricted |
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Expression was detected in the ventral midline.
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TS26: cerebellum |
Present |
|
|
|
Specimen
not shown:
embryonic day 14.0
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 14.0
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
not shown:
postnatal day 0
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
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Age:
|
postnatal day 0
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
not shown:
postnatal day 12
(more )
Specimen
not shown:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
postnatal day 12
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
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