Probe:	M33 cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: brain	Trace			The authors state that expression was very close to background levels.

Structure	Level	Pattern	Image	Note
TS21: cerebral cortex ventricular layer	Strong		1C
TS21: epithalamus	Present	Regionally restricted	1C	Expression is in a dorsal epithalamic subset of progenitor cells.
TS21: cerebellum	Present		1C
TS21: hypothalamus	Present		1C
TS21: midbrain	Present	Regionally restricted	1C	Expression is in the tegmentum.
TS21: midbrain roof plate	Present	Regionally restricted	1C	Expression is in the tectum.

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	1H	Expression is in both germinal zones of the anterior telencephalon: the dorsal and medial neuroepithelium of the pallium, as well as the ventral basal ganglia.

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Present	Regionally restricted	1I	Expression is in the posterior telencephalic neuroepithelia.
TS21: forebrain	Present	Regionally restricted	1I	Expression is in the developing hippocampus.
TS21: epithalamus	Present	Regionally restricted	1I	Expression is in a dorsal epithalamic subset of progenitor cells.

Structure	Level	Pattern	Image	Note
TS21: cortical plate	Weak		1L	Expression is in post-mitotic cells of the cortical plate.

Structure	Level	Pattern	Image	Note
TS21: forebrain	Present	Regionally restricted	1O	Expression is in the developing hippocampus.

Structure	Level	Pattern	Image	Note
TS21: cerebellum	Present		1R
TS21: midbrain	Strong	Regionally restricted	1R	Expression is in the inferior colliculus.

Structure	Level	Pattern	Image	Note
TS26: cerebral cortex ventricular zone	Weak		4G' , 4G" , 4G
TS26: cortical plate	Weak		4G' , 4G" , 4G
TS26: telencephalon	Weak	Regionally restricted	4G , 4G' , 4G"	Expression is in the basal ganglia.

Structure	Level	Pattern	Image	Note
TS26: hippocampus	Present	Regionally restricted	5D	Expression is in CA1-3. Higher magnification was shown in the inset.
TS26: dentate gyrus	Present		5D	Higher magnification was shown in the inset.

Structure	Level	Pattern	Image	Note
TS26: main olfactory bulb	Present	Regionally restricted	6A	Higher magnification was shown in the inset. Expression is in neuroepithelial and granular cells in the ventricular zone and the granular layer of the bulbus and is weak in the mitral layer.

Structure	Level	Pattern	Image	Note
TS26: cerebellum	Present	Regionally restricted	7I , 7I'	Expression is in the purkinje cell layer and the external granule cell layer and is absent in the molecular layer.
TS26: midbrain	Present	Regionally restricted	7I' , 7I	Expression is in the superior and inferior colliculi.

Structure	Level	Pattern	Image	Note
TS26: rostral migratory stream	Present		8A

Structure	Level	Pattern	Image	Note
TS26: rostral migratory stream	Present		8D

Structure	Level	Pattern	Image	Note
TS27: rostral migratory stream	Present