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Gene Expression Data
Assay Details
Assay
Reference: J:98919 Cobb J, et al., Comparative analysis of genes downstream of the Hoxd cluster in developing digits and external genitalia. Development. 2005 Jul;132(13):3055-67
Assay type: RT-PCR
MGI Accession ID: MGI:3689945
Gene symbol: Epha3
Gene name: Eph receptor A3
Probe: Epha3-pC, Epha3-pD
Assay notes: The data for this real-time RT-PCR experiment was presented graphically. The probe sequence is listed in MGI:3693344.
Results
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Amount Genetic BackgroundMutant Allele(s)Sex
Lane 1 E11.5 TS19: forelimb bud Present Not Specified; total RNA involves: 129S2/SvPas * C57BL/6 Not Specified
Lane 2 E11.5 TS19: forelimb bud Present Not Specified; total RNA involves: 129S2/SvPas * C57BL/6 Hoxdtm21(Hoxd11)Ddu/Hoxdtm21(Hoxd11)DduNot Specified
Lane 3 E12.5 TS20: forelimb Present Not Specified; total RNA involves: 129S2/SvPas * C57BL/6 Not Specified
Lane 4 E12.5 TS20: forelimb Present (b) Not Specified; total RNA involves: 129S2/SvPas * C57BL/6 Hoxdtm21(Hoxd11)Ddu/Hoxdtm21(Hoxd11)DduNot Specified
Lane 5 E13.5 TS21: forelimb Present Not Specified; total RNA involves: 129S2/SvPas * C57BL/6 Not Specified
Lane 6 E13.5 TS21: forelimb Present Not Specified; total RNA involves: 129S2/SvPas * C57BL/6 Hoxdtm21(Hoxd11)Ddu/Hoxdtm21(Hoxd11)DduNot Specified
Lane 7 E14.5 TS22: forelimb Present Not Specified; total RNA involves: 129S2/SvPas * C57BL/6 Not Specified
Lane 8 E14.5 TS22: forelimb Present Not Specified; total RNA involves: 129S2/SvPas * C57BL/6 Hoxdtm21(Hoxd11)Ddu/Hoxdtm21(Hoxd11)DduNot Specified
Notes:
(a) Expression levels were increased in the mutant compared to wild type.
(b) 1.73-fold increase in expression level in E12.5 mutant forelimb.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/10/2024
MGI 6.24 		The Jackson Laboratory