Gene Expression Data
Assay Details
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Assay
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Reference:
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J:116141
Macfadyen J, et al., Endosialin is expressed on stromal fibroblasts and CNS pericytes in mouse embryos and is downregulated during development. Gene Expr Patterns. 2007 Jan;7(3):363-9
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3697809
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Gene symbol:
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Cd248
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Gene name:
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CD248 antigen, endosialin
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Antibody:
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P13
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Detection system:
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Secondary antibody coupled to Alexa Fluor 555
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Results
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Specimen
not shown:
embryonic day 15.0
(more  )
Specimen
not shown:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: blood vessel |
Present |
Regionally restricted |
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Expression in stromal fibroblasts and developing vasculature.
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| TS23: embryo mesenchyme |
Present |
Regionally restricted |
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Expression in stromal fibroblasts and developing vasculature.
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Specimen
4, endosialin:
embryonic day 15.0
(more )
Note: Double labeled: red - Cd248; green - Pecam1 (assay MGI:3697811).
Specimen
4, endosialin:
(close )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Cd248; green - Pecam1 (assay MGI:3697811).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: blood vessel |
Present |
Regionally restricted |
4_overlay (upper)
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4_endosialin
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Expression in pericytes in the vasculature, i.e. in cells tightly surrounding the endothelium, but not in endothelial cells. Higher magnification was shown in the inset.
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| TS23: brain |
Present |
Regionally restricted |
4_overlay (upper)
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4_endosialin
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Expression in pericytes in the vasculature, i.e. in cells tightly surrounding the endothelium, but not in endothelial cells. Higher magnification was shown in the inset.
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Specimen
5, endosialin:
postnatal day 4
(more )
Specimen
5, endosialin:
(close )
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Genetic Background:
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C57BL/6
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Age:
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postnatal day 4
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: blood vessel |
Moderate |
Regionally restricted |
5_Endosialin (lower)
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5_Endosialin (upper)
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Expression in pericytes covering the entire length of blood vessels from the optic nerve to the leading edge of the neovasculature. No expression in developing retinal vasculature.
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| TS28: retina |
Moderate |
Regionally restricted |
5_Endosialin (lower)
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5_Endosialin (upper)
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Expression in pericytes covering the entire length of blood vessels from the optic nerve to the leading edge of the neovasculature. No expression in developing retinal vasculature.
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