Gene Expression Data
Assay Details
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Assay
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Reference:
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J:114758
Alcaraz WA, et al., Zfp423 controls proliferation and differentiation of neural precursors in cerebellar vermis formation. Proc Natl Acad Sci U S A. 2006;103(51):19424-19429
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3716184
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Gene symbol:
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Ebf1
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Gene name:
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early B cell factor 1
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Probe:
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Ebf1 probe3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Specimens were fixed in either 4% paraformaldehyde or 10% formalin, and embedded in 1:1 OCT:Aquamount.
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Results
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Specimen
5A +/+ E12.5:
embryonic day 12.5
(more  )
Specimen
5A +/+ E12.5:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: cerebellum primordium |
Present |
Regionally restricted |
5A +/+ E12.5
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Selective expression in newly postmitotic ventricular zone-derived neurons (presumptive Purkinje cells).
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Specimen
5A nur12 E12.5:
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6 * C3H
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Mutant Allele(s):
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Zfp423nur12/Zfp423nur12
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Specimen
5A +/+ E14.5 i:
embryonic day 14.5
(more )
Specimen
5A +/+ E14.5 i:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cerebellum |
Present |
Regionally restricted |
5A +/+ E14.5 i
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Selective expression in newly postmitotic ventricular zone-derived neurons (presumptive Purkinje cells).
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Specimen
5A +/+ E14.5 ii:
embryonic day 14.5
(more )
Specimen
5A +/+ E14.5 ii:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cerebellum |
Present |
Regionally restricted |
5A +/+ E14.5 ii
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Selective expression in newly postmitotic ventricular zone-derived neurons (presumptive Purkinje cells).
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Specimen
5A nur12 E14.5 i:
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6 * C3H
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Mutant Allele(s):
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Zfp423nur12/Zfp423nur12
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cerebellum |
Present |
Regionally restricted |
5A nur12 E14.5 i
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Mediolateral pattern of expression is both increased and more diffuse than in littermate control.
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Specimen
5A nur12 E14.5 ii:
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6 * C3H
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Mutant Allele(s):
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Zfp423nur12/Zfp423nur12
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cerebellum |
Present |
Regionally restricted |
5A nur12 E14.5 ii
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Mediolateral pattern of expression is both increased and more diffuse than in littermate control.
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Specimen
5B +/+:
embryonic day 14.5
(more )
Specimen
5B +/+:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cerebellum |
Present |
Regionally restricted |
5B +/+
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Selective expression in newly postmitotic ventricular zone-derived neurons (presumptive Purkinje cells).
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Specimen
5B nur12:
(close )
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Genetic Background:
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involves: BALB/c * C57BL/6 * C3H
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Mutant Allele(s):
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Zfp423nur12/Zfp423nur12
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cerebellum |
Present |
Regionally restricted |
5B nur12
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Expressing cells in the ventricular zone are approximately twice as frequent as in littermate controls.
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Analysis Tools