Probe:	MH866 probe 774
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS22: tongue	Weak	Regionally restricted	5_Fzd2
TS22: face	Weak	Regionally restricted	5_Fzd2	Expression was weak in Face/Soft Tissues.
TS22: tail	Weak	Regionally restricted	5_Fzd2
TS22: forelimb	Weak	Regionally restricted	5_Fzd2
TS22: hindlimb	Weak	Regionally restricted	5_Fzd2
TS22: genital tubercle	Weak	Regionally restricted	5_Fzd2
TS22: skin	Absent		5_Fzd2
TS22: lung	Present	Regionally restricted	5_Fzd2
TS22: metanephros	Weak	Regionally restricted	5_Fzd2
TS22: salivary gland	Weak	Regionally restricted	5_Fzd2
TS22: tooth	Weak	Regionally restricted	5_Fzd2
TS22: vibrissa follicle	Present	Regionally restricted	5_Fzd2
TS22: esophagus	Absent		5_Fzd2
TS22: stomach	Weak	Regionally restricted	5_Fzd2
TS22: intestine	Weak	Regionally restricted	5_Fzd2
TS22: cochlea	Weak	Regionally restricted	5_Fzd2
TS22: inner ear labyrinth	Weak	Regionally restricted	5_Fzd2
TS22: olfactory epithelium	Absent		5_Fzd2
TS22: retina	Weak	Regionally restricted	5_Fzd2
TS22: basal forebrain	Absent		5_Fzd2
TS22: piriform cortex	Weak	Regionally restricted	5_Fzd2
TS22: amygdala	Absent		5_Fzd2
TS22: pretectum	Weak	Regionally restricted	5_Fzd2
TS22: dorsal root ganglion	Absent		5_Fzd2
TS22: cranial ganglion	Absent		5_Fzd2
TS22: corpus striatum	Present	Regionally restricted	5_Fzd2
TS22: pallidum	Present	Regionally restricted	5_Fzd2
TS22: midbrain tegmentum	Strong	Regionally restricted	5_Fzd2
TS22: spinal cord	Present	Regionally restricted	5_Fzd2
TS22: pons	Strong	Regionally restricted	5_Fzd2
TS22: medulla oblongata	Strong	Regionally restricted	5_Fzd2
TS22: telencephalon septum	Present	Regionally restricted	5_Fzd2
TS22: thalamus	Present	Regionally restricted	5_Fzd2
TS22: hypothalamus	Present	Regionally restricted	5_Fzd2
TS22: cerebellum	Present	Regionally restricted	5_Fzd2
TS22: superior colliculus	Present	Regionally restricted	5_Fzd2
TS22: inferior colliculus	Present	Regionally restricted	5_Fzd2
TS22: main olfactory bulb	Present	Regionally restricted	5_Fzd2
TS22: neocortex	Present	Regionally restricted	5_Fzd2
TS22: hippocampus	Present	Regionally restricted	5_Fzd2
TS22: ureter	Absent		5_Fzd2
TS22: bladder	Absent		5_Fzd2
TS22: urethra	Absent		5_Fzd2
TS22: main bronchus	Weak	Regionally restricted	5_Fzd2
TS22: trachea	Weak	Regionally restricted	5_Fzd2
TS22: palatal shelf	Weak	Regionally restricted	5_Fzd2
TS22: middle ear	Absent		5_Fzd2
TS22: pharynx	Absent		5_Fzd2
TS22: larynx	Absent		5_Fzd2
TS22: optic nerve	Absent		5_Fzd2
TS22: liver	Absent		5_Fzd2
TS22: thymus primordium	Weak	Regionally restricted	5_Fzd2
TS22: thyroid gland	Weak	Regionally restricted	5_Fzd2
TS22: pituitary gland	Weak	Regionally restricted	5_Fzd2
TS22: pancreas	Weak	Regionally restricted	5_Fzd2
TS22: spleen primordium	Absent		5_Fzd2	Expression was absent in Spleen.
TS22: adrenal gland	Absent		5_Fzd2
TS22: gonad	Weak	Regionally restricted	5_Fzd2
TS22: musculature	Weak	Regionally restricted	5_Fzd2	Expression was weak in Muscle System.
TS22: choroid plexus	Weak	Regionally restricted	5_Fzd2
TS22: meninges	Absent		5_Fzd2
TS22: blood vessel	Weak	Regionally restricted	5_Fzd2
TS22: heart atrium	Weak	Regionally restricted	5_Fzd2
TS22: heart ventricle	Weak	Regionally restricted	5_Fzd2
TS22: appendicular skeleton	Absent		5_Fzd2
TS22: axial skeleton	Absent		5_Fzd2
TS22: cranium	Weak	Regionally restricted	5_Fzd2
TS22: facial bone primordium	Weak	Regionally restricted	5_Fzd2
TS22: lens	Weak	Regionally restricted	5_Fzd2
TS22: cornea	Absent		5_Fzd2

Structure	Level	Pattern	Image	Note
TS22: telencephalon ventricular layer	Present		6C

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present		7H

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Present		8N1	Expression was widespread in the cortex.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Present		8N2	Expression intensity was reduced in the mutant compared to wild type.