Probe:	MH740 probe 657
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS22: tongue	Present	Regionally restricted	5_Arx
TS22: face	Present	Regionally restricted	5_Arx	Expression was present in Face/Soft Tissues.
TS22: tail	Strong	Regionally restricted	5_Arx
TS22: forelimb	Weak	Regionally restricted	5_Arx
TS22: hindlimb	Present	Regionally restricted	5_Arx
TS22: genital tubercle	Absent		5_Arx
TS22: skin	Present	Regionally restricted	5_Arx
TS22: lung	Absent		5_Arx
TS22: metanephros	Absent		5_Arx
TS22: salivary gland	Absent		5_Arx
TS22: tooth	Absent		5_Arx
TS22: vibrissa follicle	Absent		5_Arx
TS22: esophagus	Absent		5_Arx
TS22: stomach	Weak	Regionally restricted	5_Arx
TS22: intestine	Absent		5_Arx
TS22: cochlea	Absent		5_Arx
TS22: inner ear labyrinth	Absent		5_Arx
TS22: olfactory epithelium	Absent		5_Arx
TS22: retina	Absent		5_Arx
TS22: basal forebrain	Present	Regionally restricted	5_Arx
TS22: piriform cortex	Present	Regionally restricted	5_Arx
TS22: amygdala	Present	Regionally restricted	5_Arx
TS22: pretectum	Absent		5_Arx
TS22: dorsal root ganglion	Absent		5_Arx
TS22: cranial ganglion	Absent		5_Arx
TS22: corpus striatum	Present	Regionally restricted	5_Arx
TS22: pallidum	Present	Regionally restricted	5_Arx
TS22: midbrain tegmentum	Strong	Regionally restricted	5_Arx
TS22: spinal cord	Strong	Regionally restricted	5_Arx
TS22: pons	Strong	Regionally restricted	5_Arx
TS22: medulla oblongata	Strong	Regionally restricted	5_Arx
TS22: telencephalon septum	Present	Regionally restricted	5_Arx
TS22: thalamus	Strong	Regionally restricted	5_Arx
TS22: hypothalamus	Strong	Regionally restricted	5_Arx
TS22: cerebellum	Weak	Regionally restricted	5_Arx
TS22: superior colliculus	Absent		5_Arx
TS22: inferior colliculus	Absent		5_Arx
TS22: main olfactory bulb	Present	Regionally restricted	5_Arx
TS22: neocortex	Present	Regionally restricted	5_Arx
TS22: hippocampus	Present	Regionally restricted	5_Arx
TS22: ureter	Absent		5_Arx
TS22: bladder	Weak	Regionally restricted	5_Arx
TS22: urethra	Absent		5_Arx
TS22: main bronchus	Absent		5_Arx
TS22: trachea	Absent		5_Arx
TS22: palatal shelf	Absent		5_Arx
TS22: middle ear	Absent		5_Arx
TS22: pharynx	Absent		5_Arx
TS22: larynx	Absent		5_Arx
TS22: optic nerve	Absent		5_Arx
TS22: liver	Absent		5_Arx
TS22: thymus primordium	Absent		5_Arx
TS22: thyroid gland	Absent		5_Arx
TS22: pituitary gland	Absent		5_Arx
TS22: pancreas	Strong	Regionally restricted	5_Arx
TS22: spleen primordium	Absent		5_Arx	Expression was absent in Spleen.
TS22: adrenal gland	Absent		5_Arx
TS22: gonad	Present	Regionally restricted	5_Arx
TS22: musculature	Present	Regionally restricted	5_Arx	Expression was present in Muscle System.
TS22: choroid plexus	Weak	Regionally restricted	5_Arx
TS22: meninges	Weak	Regionally restricted	5_Arx
TS22: blood vessel	Absent		5_Arx
TS22: heart atrium	Absent		5_Arx
TS22: heart ventricle	Absent		5_Arx
TS22: appendicular skeleton	Weak	Regionally restricted	5_Arx
TS22: axial skeleton	Weak	Regionally restricted	5_Arx
TS22: cranium	Weak	Regionally restricted	5_Arx
TS22: facial bone primordium	Weak	Regionally restricted	5_Arx
TS22: lens	Weak	Regionally restricted	5_Arx

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex	Present		7C

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Present	Patchy	8D1	Expression in neurons was scattered at every level of the developing cortex, particularly in the marginal zone.
TS23: cerebral cortex marginal layer	Present	Patchy	8D1	Expression in neurons was scattered at every level of the developing cortex, particularly in the marginal zone.

Structure	Level	Pattern	Image	Note
TS23: cerebral cortex	Present	Patchy	8D2	Expression in neurons was scattered at every level of the developing cortex, particularly in the marginal zone as in wild type.
TS23: cerebral cortex marginal layer	Present	Patchy	8D2	Expression in neurons was scattered at every level of the developing cortex, particularly in the marginal zone as in wild type.
TS23: cerebral cortex subventricular zone	Present		8D2	A novel expression domain was detected coinciding with the subventricular zone of the mutant cortex.