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Gene Expression Data
Assay Details
Assay
Reference: J:125993 Cankaya M, et al., An analysis of expression patterns of genes encoding proteins with catalytic activities. BMC Genomics. 2007;8:232
Assay type: RNA in situ
MGI Accession ID: MGI:3769766
Gene symbol: Pgk1
Gene name: phosphoglycerate kinase 1
Probe: MH2060 probe 1821
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.
Results
Specimen 3A: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: neocortex Moderate 3A Pgk1
TS22: midbrain Moderate 3A Pgk1
TS22: cerebellum Moderate 3A Pgk1
TS22: pons Moderate 3A Pgk1
TS22: medulla oblongata Moderate 3A Pgk1
TS22: spinal cord Moderate 3A Pgk1
TS22: cranial ganglion Moderate 3A Pgk1
TS22: dorsal root ganglion Moderate 3A Pgk1
TS22: olfactory epithelium Moderate 3A Pgk1
TS22: intestine Moderate 3A Pgk1
TS22: salivary gland Moderate 3A Pgk1
TS22: tongue Moderate 3A Pgk1
TS22: stomach Moderate 3A Pgk1
TS22: liver Moderate 3A Pgk1
TS22: lung Moderate 3A Pgk1
TS22: metanephros Moderate 3A Pgk1
TS22: thyroid gland Moderate 3A Pgk1
TS22: pituitary gland Moderate 3A Pgk1
TS22: pancreas Moderate 3A Pgk1
TS22: adrenal gland Moderate 3A Pgk1
TS22: heart Moderate 3A Pgk1
TS22: skeletal muscle Weak 3A Pgk1
TS22: cartilage Absent 3A Pgk1 There is no expression in the axial cartilage.
TS22: skeleton Absent 3A Pgk1 There is no expression in the long bones.
Specimen MH2060: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: integumental system Strong MH2060_1D , MH2060_5B There is strong expression throughout the integuments.
TS22: embryo Present Regionally restricted MH2060_1D , MH2060_5B Expression is found in all organs, with the exception of the axial skeleton.
TS22: axial skeleton Absent MH2060_5B , MH2060_1D
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/10/2024
MGI 6.24 		The Jackson Laboratory