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Gene Expression Data
Assay Details
Assay
Reference: J:125993 Cankaya M, et al., An analysis of expression patterns of genes encoding proteins with catalytic activities. BMC Genomics. 2007;8:232
Assay type: RNA in situ
MGI Accession ID: MGI:3769782
Gene symbol: Pkm
Gene name: pyruvate kinase, muscle
Probe: MH1052 probe 913
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Assay notes: Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 15-30 min. before further processing. A dual signal amplification strategy, termed catalyzed reporter deposition (CARD), was used for detection.
Results
Specimen 3A: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: neocortex Strong 3A Pkm2
TS22: midbrain Moderate 3A Pkm2
TS22: cerebellum Moderate 3A Pkm2
TS22: pons Moderate 3A Pkm2
TS22: medulla oblongata Moderate 3A Pkm2
TS22: spinal cord Strong 3A Pkm2
TS22: cranial ganglion Moderate 3A Pkm2
TS22: dorsal root ganglion Moderate 3A Pkm2
TS22: olfactory epithelium Strong 3A Pkm2
TS22: intestine Strong 3A Pkm2
TS22: salivary gland Strong 3A Pkm2
TS22: tongue Moderate 3A Pkm2
TS22: stomach Strong 3A Pkm2
TS22: liver Moderate 3A Pkm2
TS22: lung Strong 3A Pkm2
TS22: metanephros Strong 3A Pkm2
TS22: thyroid gland Strong 3A Pkm2
TS22: pituitary gland Strong 3A Pkm2
TS22: pancreas Strong 3A Pkm2
TS22: adrenal gland Moderate 3A Pkm2
TS22: heart Strong 3A Pkm2
TS22: skeletal muscle Strong 3A Pkm2
TS22: cartilage Absent 3A Pkm2 There is no expression in the axial cartilage.
TS22: skeleton Absent 3A Pkm2 There is no expression in the long bones.
Specimen MH1052: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: central nervous system Present Non-Uniform MH1052_4A , MH1052_2D Expression is ubiquitous in the central nervous system but is elevated in certain structures.
TS22: brain ventricular layer Present Non-Uniform MH1052_4A , MH1052_2D Expression is elevated in the ventricular zone that lines the left ventricles.
TS22: inferior colliculus Present MH1052_2D , MH1052_4A Expression is elevated in this structure.
TS22: embryo Present Regionally restricted MH1052_4A , MH1052_2D Many tissues outside the central nervous system express this gene.
TS22: vertebral pre-cartilage condensation Absent MH1052_4A , MH1052_2D
TS22: liver Ambiguous MH1052_4A , MH1052_2D In the liver, most cells do not express, with the exception of blood vessels.
TS22: blood vessel Present MH1052_2D , MH1052_4A Expression is present in blood vessels of the liver.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Funding Information
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12/10/2024
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