Gene Expression Data
Assay Details
Assay
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Reference:
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J:85300
Helmbacher F, et al., Met signaling is required for recruitment of motor neurons to PEA3-positive motor pools. Neuron. 2003 Aug 28;39(5):767-77
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3776080
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Gene symbol:
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Etv4
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Gene name:
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ets variant 4
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Probe:
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Pea3 probe1
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Probe preparation:
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labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
5F right:
embryonic day 11.5
(more )
Note: 52 somites. Double labeled: red - Etv4; blue - Met (assay MGI:3776083).
Specimen
5F right:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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52 somites. Double labeled: red - Etv4; blue - Met (assay MGI:3776083).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: future spinal cord |
Present |
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5F right
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Expression was detected in the C7-C8 region of the spinal cord.
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Specimen
5G right:
(close )
Genetic Background:
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involves: 129S2/SvPas * CD-1
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Mutant Allele(s):
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Gdnftm1Rosl/Gdnftm1Rosl
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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52 somites. Double labeled: red - Etv4; blue - Met (assay MGI:3776083).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: future spinal cord |
Ambiguous |
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5G right
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The expression pattern for this specimen was not described.
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Specimen
5H right:
(close )
Genetic Background:
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involves: 129S1/Sv
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Mutant Allele(s):
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Etv4tm1Arbr/Etv4tm1Arbr
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
|
Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
Note:
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52 somites. Double labeled: red - Etv4; blue - Met (assay MGI:3776083).
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Specimen
6A right:
embryonic day 11.0
(more )
Note: 41 somites. Double labeled: red - Etv4; blue - Met (assay MGI:3776083).
Specimen
6A right:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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41 somites. Double labeled: red - Etv4; blue - Met (assay MGI:3776083).
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Specimen
6B right:
embryonic day 11.0
(more )
Note: 44-45 somites. Double labeled: red - Etv4; blue - Met (assay MGI:3776083).
Specimen
6B right:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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44-45 somites. Double labeled: red - Etv4; blue - Met (assay MGI:3776083).
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Specimen
6C right:
embryonic day 11.5
(more )
Note: 47-48 somites. Double labeled: red - Etv4; blue - Met (assay MGI:3776083).
Specimen
6C right:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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47-48 somites. Double labeled: red - Etv4; blue - Met (assay MGI:3776083).
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Specimen
6D right:
embryonic day 11.5
(more )
Note: 52 somites. Double labeled: red - Etv4; blue - Met (assay MGI:3776083).
Specimen
6D right:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 11.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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52 somites. Double labeled: red - Etv4; blue - Met (assay MGI:3776083).
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Specimen
S2B:
embryonic day 12.5
(more )
Specimen
S2B:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Present |
Regionally restricted |
S2B
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Expression covers slightly more than four spinal segments.
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Specimen
S2E:
embryonic day 12.5
(more )
Specimen
S2E:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
|
4% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Present |
Regionally restricted |
S2E
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Expression was detected in the C7-C8 levels of the spinal cord in more medial motor neurons.
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Specimen
S2F:
embryonic day 12.5
(more )
Note: Double labeled: red - Etv4; blue - Met (assay MGI:3776083).
Specimen
S2F:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
Note:
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Double labeled: red - Etv4; blue - Met (assay MGI:3776083).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Present |
Regionally restricted |
S2F
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Expression was detected in the C7-C8 levels of the spinal cord in more medial motor neurons.
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