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Gene Expression Data
Assay Details
Assay
Reference: J:98502 Strumpf D, et al., Cdx2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocyst. Development. 2005 May;132(9):2093-102
Assay type: Immunohistochemistry
MGI Accession ID: MGI:3777389
Gene symbol: Cdx2
Gene name: caudal type homeobox 2
Antibody: anti-Cdx2 (CNL)
Detection system: Secondary antibody
Results
Specimen 1C: embryonic day 3.0 (more )
Note: 25-cell embryo. Double labeled: red - Cdx2; green - Pou5f1 (assay MGI:3777390).
Structure Level Pattern Image Note
TS4: compacted morula Present Non-Uniform 1C , 1D Expression is restricted to the outer cells. There are high levels of expression in the nuclei and lower levels in the cytoplasm.
Specimen 1E: embryonic day 3.25 (more )
Note: This is an early blastocyst. Authors did not specify which antibody was used; it is possible MGI:3777387 was used instead.
Structure Level Pattern Image Note
TS4: blastocyst Present 1E Expression is present in the nucleus and cytoplasm, primarily in the outer cell layer.
Specimen 1F: embryonic day 3.5 (more )
Note: This is a blastocyst. Authors did not specify which antibody was used; it is possible MGI:3777387 was used instead.
Structure Level Pattern Image Note
TS4: trophectoderm Present 1F Expression is exclusively localized to the trophectoderm nuclei.
TS4: inner cell mass Absent 1F
Specimen 1G: embryonic day 4.5 (more )
Note: This is an implanting blastocyst. Authors did not specify which antibody was used; it is possible MGI:3777387 was used instead.
Structure Level Pattern Image Note
TS5: polar trophectoderm Present 1G Expression is present in polar trophectoderm cells directly overlying the inner cell mass.
TS5: mural trophectoderm Present 1G Expression levels are reduced compared to what was seen in the mural trophectoderm earlier in development.
TS5: inner cell mass Absent 1G
Specimen 1H: embryonic day 4.75 (more )
Note: Authors did not specify which antibody was used; it is possible MGI:3777387 was used instead.
Structure Level Pattern Image Note
TS6: polar trophectoderm Present Regionally restricted 1H Expression is confined to the polar trophectoderm cells directly overlaying the epiblast.
Specimen 1I: embryonic day 5.5 (more )
Note: Authors did not specify which antibody was used; it is possible MGI:3777387 was used instead.
Structure Level Pattern Image Note
TS7: extraembryonic ectoderm Strong Regionally restricted 1I There is intense expression in the first three or four cells rows in the extraembryonic ectoderm adjacent to the epiblast.
Specimen 5A: embryonic day 3.5 (more )
Note: This is an expanded blastocyst. This embryo may be a heterozygote. Double labeled: red - Cdx2; green - Pou5f1 (assay MGI:3777390). Authors did not specify which antibody was used; it is possible MGI:3777387 was used instead.
Structure Level Pattern Image Note
TS4: trophectoderm Present 5A
TS4: inner cell mass Absent 5A
Specimen 5B: embryonic day 3.5; Cdx2tm1Fbe/Cdx2tm1Fbe (more )
Note: This is a blastocyst. Double labeled: red - Cdx2; green - Pou5f1 (assay MGI:3777390). Authors did not specify which antibody was used; it is possible MGI:3777387 was used instead.
Structure Level Pattern Image Note
TS4: inner cell mass Absent 5B
TS4: trophectoderm Absent 5B
Specimen 8B: embryonic day 4.5; Eomestm1Jrt/Eomes+ (more )
Note: Double labeled: red - Cdx2; green - Pou5f1 (assay MGI:3777390). Authors did not specify which antibody was used; it is possible MGI:3777387 was used instead.
Structure Level Pattern Image Note
TS5: trophectoderm Present 8B
Specimen 8F: embryonic day 4.5; Eomestm1Jrt/Eomestm1Jrt (more )
Note: Double labeled: red - Cdx2; green - Pou5f1 (assay MGI:3777390). Authors did not specify which antibody was used; it is possible MGI:3777387 was used instead.
Structure Level Pattern Image Note
TS5: trophectoderm Present 8F
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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