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Gene Expression Data
Assay Details
Assay
Reference: J:98502 Strumpf D, et al., Cdx2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocyst. Development. 2005 May;132(9):2093-102
Assay type: Immunohistochemistry
MGI Accession ID: MGI:3777390
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Antibody: anti-Oct3/4 (C-10; sc-5279)
Detection system: Secondary antibody
Results
Specimen 1D: embryonic day 3.0 (more )
Note: 25-cell embryo. Double labeled: green - Pou5f1; red - Cdx2 (assay MGI:3777389).
Structure Level Pattern Image Note
TS4: compacted morula Present 1D Expression is present in all cells of the embryo, including in the outer cells.
Specimen 4B: embryonic day 4.5 (more )
Note: This is a blastocyst. Double labeled: red - Pou5f1; blue - Itga7 (assay MGI:3777410).
Structure Level Pattern Image Note
TS5: inner cell mass Present 4B
TS5: extraembryonic component Absent 4B Expression levels are reduced in the primitive endoderm.
Specimen 4E: embryonic day 4.5; Cdx2tm1Fbe/Cdx2tm1Fbe (more )
Note: Double labeled: red - Pou5f1; blue - Itga7 (assay MGI:3777410).
Structure Level Pattern Image Note
TS5: inner cell mass Present 4E Expression is found in all or almost all cells.
TS5: trophectoderm Present 4E Expression is found in all or almost all cells.
Specimen 5A: embryonic day 3.5 (more )
Note: This is a blastocyst. This embryo may be a heterozygote. Double labeled: green - Pou5f1; red - Cdx2 (assay MGI:3777389).
Structure Level Pattern Image Note
TS4: inner cell mass Present 5A
TS4: trophectoderm Absent 5A
Specimen 5B: embryonic day 3.5; Cdx2tm1Fbe/Cdx2tm1Fbe (more )
Note: This is a blastocyst. Double labeled: green - Pou5f1; red - Cdx2 (assay MGI:3777389).
Structure Level Pattern Image Note
TS4: inner cell mass Present 5B Expression is present in all cells of the embryo.
TS4: trophectoderm Present 5B Expression is present in all cells of the embryo.
Specimen 8B: embryonic day 4.5; Eomestm1Jrt/Eomes+ (more )
Note: Double labeled: green - Pou5f1; red - Cdx2 (assay MGI:3777389).
Structure Level Pattern Image Note
TS5: inner cell mass Present 8B
Specimen 8F: embryonic day 4.5; Eomestm1Jrt/Eomestm1Jrt (more )
Note: Double labeled: green - Pou5f1; red - Cdx2 (assay MGI:3777389).
Structure Level Pattern Image Note
TS5: inner cell mass Present 8F
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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06/12/2024
MGI 6.13 		The Jackson Laboratory