Antibody:	MCM6 antibody
Detection system:	Secondary antibody coupled to Alexa Fluor
Assay notes:	Tissue samples were fixed in 4% neutral buffered formalin.

Structure	Level	Pattern	Image	Note
TS15: optic cup	Present	Regionally restricted		Expression is in most cells of the retina and surrounding tissues.

Structure	Level	Pattern	Image	Note
TS19: lens vesicle	Present		1A , 1C
TS19: pigmented retinal epithelium	Present		1A , 1C
TS19: blood vessel	Present	Regionally restricted	1C , 1A	Expression is in embryonic vasculature in and near the eye and in the extraocular tissues.
TS19: eye	Present	Regionally restricted	1C , 1A	Expression is in embryonic vasculature in and near the eye and in the extraocular tissues.
TS19: head	Present	Regionally restricted	1C , 1A	Expression is in embryonic vasculature in and near the eye and in the extraocular tissues.

Structure	Level	Pattern	Image	Note
TS21: pigmented retinal epithelium	Present		1D , 1F
TS21: retina	Present		1D , 1F
TS21: blood vessel	Present	Regionally restricted	1D , 1F	Expression is in embryonic vasculature in and near the eye and in the extraocular tissues.
TS21: eye	Present	Regionally restricted	1D , 1F	Expression is in embryonic vasculature in and near the eye and in the extraocular tissues.
TS21: head	Present	Regionally restricted	1D , 1F	Expression is in embryonic vasculature in and near the eye and in the extraocular tissues.

Structure	Level	Pattern	Image	Note
TS24: retina nuclear layer	Present	Regionally restricted	1I , 1G	Expression is in the neuroblast layer and excluded from cells located in the developing ganglion cell layer and inner boundary of the neuroblastic layer.

Structure	Level	Pattern	Image	Note
TS27: retina layer	Present	Regionally restricted	1L , 1J	Expression is in the neuroblast layer and excluded from cells located in the developing ganglion cell layer and inner boundary of the neuroblastic layer.

Structure	Level	Pattern	Image	Note
TS28: retina layer	Present	Regionally restricted	1O , 1M	Expression is in a narrow band of cells in the developing inner nuclear layer.

Structure	Level	Pattern	Image	Note
TS28: retina layer	Absent		1P , 1R

Structure	Level	Pattern	Image	Note
TS21: eye	Present		4C , 4A
TS21: retina	Weak	Regionally restricted	4A , 4C	Weak expression in the inner retina.

Structure	Level	Pattern	Image	Note
TS27: retina layer	Present	Regionally restricted	4F , 4D	Expression is in the neuroblast layer in newly postmitotic cells.

Structure	Level	Pattern	Image	Note
TS27: retina layer	Present	Regionally restricted	4G , 4I	Expression is in the neuroblast layer in newly postmitotic cells.

Structure	Level	Pattern	Image	Note
TS27: retina layer	Present	Regionally restricted	4L , 4J	Expression is in the neuroblast layer in newly postmitotic cells.
TS27: photoreceptor layer	Weak	Single cells	4J , 4L

Structure	Level	Pattern	Image	Note
TS27: retina layer	Present	Regionally restricted	4M , 4O	Expression is in the neuroblast layer in newly postmitotic cells.

Structure	Level	Pattern	Image	Note
TS27: retina layer	Present	Regionally restricted	7C , 7A	Expression is in the neuroblast layer.

Structure	Level	Pattern	Image	Note
TS27: retina layer	Present	Regionally restricted	7D , 7F	Expression is in the neuroblast layer.

Structure	Level	Pattern	Image	Note
TS27: retina layer	Present	Regionally restricted	7G , 7I	Expression is in the neuroblast layer.

Structure	Level	Pattern	Image	Note
TS27: retina layer	Present	Regionally restricted	7L , 7J	Expression is in the neuroblast layer.

Structure	Level	Pattern	Image	Note
TS27: retina layer	Present	Regionally restricted	7M , 7O	Expression is in the neuroblast layer.