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Assay
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Reference:
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J:132131
Barton KM, et al., Expression patterns and cell cycle profiles of PCNA, MCM6, cyclin D1, cyclin A2, cyclin B1, and phosphorylated histone H3 in the developing mouse retina. Dev Dyn. 2008 Mar;237(3):672-82
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:3784955
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Gene symbol:
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Pcna
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Gene name:
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proliferating cell nuclear antigen
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Antibody:
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PC10
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Assay notes:
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Tissue
samples were fixed in 4% neutral
buffered formalin.
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Results
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Specimen
Not shown:
embryonic day 9.5
(more  )
Specimen
Not shown:
(close  )
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Genetic Background:
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either: Black Swiss or Swiss Webster
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Age:
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embryonic day 9.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: optic cup |
Present |
Regionally restricted |
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Expression is in most cells of the retina and surrounding tissues.
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Specimen
1B,C:
embryonic day 11.5
(more )
Note: Double labeled: red - Pcna; green - Mcm6 (assay MGI:3784865).
Specimen
1B,C:
(close )
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Genetic Background:
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either: Black Swiss or Swiss Webster
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Age:
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embryonic day 11.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pcna; green - Mcm6 (assay MGI:3784865).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: lens vesicle |
Present |
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1B
,
1C
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| TS19: pigmented retinal epithelium |
Present |
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1B
,
1C
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| TS19: blood vessel |
Present |
Regionally restricted |
1B
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1C
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Expression is in embryonic vasculature in and near the eye and in the extraocular tissues.
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| TS19: eye |
Present |
Regionally restricted |
1B
,
1C
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Expression is in embryonic vasculature in and near the eye and in the extraocular tissues.
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| TS19: head |
Present |
Regionally restricted |
1B
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1C
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Expression is in embryonic vasculature in and near the eye and in the extraocular tissues.
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Specimen
1E,F:
embryonic day 13.5
(more )
Note: Double labeled: red - Pcna; green - Mcm6 (assay MGI:3784865).
Specimen
1E,F:
(close )
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Genetic Background:
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either: Black Swiss or Swiss Webster
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pcna; green - Mcm6 (assay MGI:3784865).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: pigmented retinal epithelium |
Present |
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1F
,
1E
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| TS21: retina |
Present |
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1F
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1E
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| TS21: blood vessel |
Present |
Regionally restricted |
1E
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1F
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Expression is in embryonic vasculature in and near the eye and in the extraocular tissues.
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| TS21: eye |
Present |
Regionally restricted |
1E
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1F
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Expression is in embryonic vasculature in and near the eye and in the extraocular tissues.
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| TS21: head |
Present |
Regionally restricted |
1E
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1F
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Expression is in embryonic vasculature in and near the eye and in the extraocular tissues.
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Specimen
1H,I:
embryonic day 16.5
(more )
Note: Double labeled: red - Pcna; green - Mcm6 (assay MGI:3784865).
Specimen
1H,I:
(close )
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Genetic Background:
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either: Black Swiss or Swiss Webster
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Age:
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embryonic day 16.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pcna; green - Mcm6 (assay MGI:3784865).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: retina nuclear layer |
Present |
Regionally restricted |
1H
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1I
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Expression is in the neuroblast layer and excluded from cells located in the developing ganglion cell layer and inner boundary of the neuroblastic layer.
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Specimen
1K,L:
postnatal day 3
(more )
Note: Double labeled: red - Pcna; green - Mcm6 (assay MGI:3784865).
Specimen
1K,L:
(close )
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Genetic Background:
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either: Black Swiss or Swiss Webster
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Age:
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postnatal day 3
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pcna; green - Mcm6 (assay MGI:3784865).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: retina layer |
Present |
Regionally restricted |
1K
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1L
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Expression is in the neuroblast layer and excluded from cells located in the developing ganglion cell layer and inner boundary of the neuroblastic layer.
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Specimen
1N,O:
postnatal day 6
(more )
Note: Double labeled: red - Pcna; green - Mcm6 (assay MGI:3784865).
Specimen
1N,O:
(close )
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Genetic Background:
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either: Black Swiss or Swiss Webster
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Age:
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postnatal day 6
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pcna; green - Mcm6 (assay MGI:3784865).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: retina inner nuclear layer |
Present |
Regionally restricted |
1N
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1O
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Expression is in a narrow band of cells in the developing inner nuclear layer.
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Specimen
1Q,R:
postnatal day 15
(more )
Note: Double labeled: red - Pcna; green - Mcm6 (assay MGI:3784865).
Specimen
1Q,R:
(close )
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Genetic Background:
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either: Black Swiss or Swiss Webster
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Age:
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postnatal day 15
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pcna; green - Mcm6 (assay MGI:3784865).
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Specimen
5A,C:
embryonic day 13.5
(more )
Note: Double labeled: green - Pcna; red - Tubb3 (assay MGI:3784956)
Specimen
5A,C:
(close )
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Genetic Background:
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either: Black Swiss or Swiss Webster
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Pcna; red - Tubb3 (assay MGI:3784956)
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Specimen
5D,F:
postnatal day 0
(more )
Note: Double labeled: green - Pcna; red - Tubb3 (assay MGI:3784956)
Specimen
5D,F:
(close )
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Genetic Background:
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either: Black Swiss or Swiss Webster
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Pcna; red - Tubb3 (assay MGI:3784956)
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: retina layer |
Present |
Regionally restricted |
5D
,
5F
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Expression is in the neuroblast layer.
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Specimen
5G,I:
postnatal day 0
(more )
Note: Double labeled: green - Pcna; red - Nefm (assay MGI:3784957).
Specimen
5G,I:
(close )
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Genetic Background:
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either: Black Swiss or Swiss Webster
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Pcna; red - Nefm (assay MGI:3784957).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: retina layer |
Present |
Regionally restricted |
5G
,
5I
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Expression is in the neuroblast layer.
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Specimen
5J,L:
postnatal day 0
(more )
Note: Double labeled: green - Pcna; red - Rcvrn (assay MGI:3784958).
Specimen
5J,L:
(close )
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Genetic Background:
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either: Black Swiss or Swiss Webster
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Pcna; red - Rcvrn (assay MGI:3784958).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: retina layer |
Present |
Regionally restricted |
5J
,
5L
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Expression is in the neuroblast layer.
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Specimen
7P,R:
postnatal day 0
(more )
Note: Double labeled: green - Pcna; red - pHH3.
Specimen
7P,R:
(close )
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Genetic Background:
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either: Black Swiss or Swiss Webster
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Cryosection
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Note:
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Double labeled: green - Pcna; red - pHH3.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: retina layer |
Present |
Regionally restricted |
7R
,
7P
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Expression is in the neuroblast layer.
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Analysis Tools