Gene Expression Data
Assay Details
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Assay
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Reference:
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J:108509
Blaess S, et al., Sonic hedgehog regulates Gli activator and repressor functions with spatial and temporal precision in the mid/hindbrain region. Development. 2006 May;133(9):1799-809
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3810934
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Gene symbol:
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Gli3
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Gene name:
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GLI-Kruppel family member GLI3
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Results
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Specimen
5K:
embryonic day 9.5
(more  )
Specimen
5K:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future midbrain |
Present |
Regionally restricted |
5K
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Expression is present in the dorsolateral mesencephalon.
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Specimen
5L:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Swiss Webster
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Mutant Allele(s):
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Smotm2Amc/Smotm2Amc
En1tm2(cre)Wrst/En1+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future midbrain |
Present |
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5L
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Expression is extended more ventrally in the mutants.
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Specimen
Not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Swiss Webster
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Mutant Allele(s):
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Shhtm1Chg/Shhtm1Chg
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future midbrain |
Present |
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Expression is extended more ventrally in the mutants.
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Specimen
5M:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Swiss Webster
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Mutant Allele(s):
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Gli2tm1Alj/Gli2tm1Alj
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future midbrain |
Present |
Graded |
5M
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Expression is extended more ventrally in the mutants, albeit in a dorsal-to-ventral gradient.
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Specimen
5N:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S6/SvEvTac * 129X1/SvJ * Swiss Webster
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Mutant Allele(s):
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Gli2tm6Alj/Gli2tm6Alj
En1tm2(cre)Wrst/En1+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future midbrain |
Present |
Graded |
5N
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Expression is extended more ventrally in the mutants, albeit in a dorsal-to-ventral gradient.
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Specimen
5O:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL * Swiss Webster
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Mutant Allele(s):
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Smotm1Amc/Smotm2Amc
Tg(Nes-cre)1Kln/0
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Conditional mutant.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: midbrain |
Present |
Regionally restricted |
5O
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Expression is present in the dorsolateral mesencephalon similar to wild type.
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Analysis Tools