Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:44351
Chen L, et al., Molecular cloning and analysis of novel cDNAs specifically expressed in adult mouse testes. Biochem Biophys Res Commun. 1997 Nov 17;240(2):261-8
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:3812375
|
Gene symbol:
|
Tex9
|
Gene name:
|
testis expressed gene 9
|
Probe:
|
T31 probe
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
Visualized with:
|
Alkaline phosphatase
|
|
Results
|
Specimen
5a, 5b:
postnatal week 10
(more )
Note: Fig. 5b and 5b inset are higher magnifications of 5a.
Specimen
5a, 5b:
(close )
Genetic Background:
|
ICR
|
Age:
|
postnatal week 10
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Fig. 5b and 5b inset are higher magnifications of 5a.
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS28: seminiferous tubule |
|
Present |
Regionally restricted |
5a
|
Expression was detected in the outer layer of seminiferous tubules.
|
TS28: spermatogonium |
|
Present |
|
5b inset
,
5a
,
5b
|
Expression was detected in spermatogonia and Sertoli cells located adjacent to the basement membrane of the seminiferous tubules and in Leydig cells. No differentiating spermatocytes or spermatids hybridized with the probe.
|
TS28: Leydig cell |
Leydig cell |
Present |
|
5b inset
,
5b
,
5a
|
|
TS28: Sertoli cell |
Sertoli cell |
Present |
|
5b
,
5a
,
5b inset
|
|
TS28: spermatid |
|
Absent |
|
5b inset
,
5a
,
5b
|
Expression was detected in spermatogonia and Sertoli cells located adjacent to the basement membrane of the seminiferous tubules and in Leydig cells. No differentiating spermatocytes or spermatids hybridized with the probe.
|
TS28: spermatocyte |
|
Absent |
|
5b inset
,
5a
,
5b
|
Expression was detected in spermatogonia and Sertoli cells located adjacent to the basement membrane of the seminiferous tubules and in Leydig cells. No differentiating spermatocytes or spermatids hybridized with the probe.
|
Specimen
5d:
postnatal week 12; KitW/ KitW-v
(more )
Specimen
5d:
(close )
Genetic Background:
|
Not Specified
|
Mutant Allele(s):
|
KitW/KitW-v
|
Age:
|
postnatal week 12
|
Sex:
|
Male
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
|