Gene Expression Data
Assay Details
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Assay
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Reference:
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J:139177
Hoffman BG, et al., Identification of transcripts with enriched expression in the developing and adult pancreas. Genome Biol. 2008;9(6):R99
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3825890
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Gene symbol:
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Smurf1
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Gene name:
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SMAD specific E3 ubiquitin protein ligase 1
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Probe:
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MGC clone 28022
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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Specimen information for this assay was downloaded from GenePaint (www.genepaint.org). Cryosections of fresh frozen material were fixed in 4% paraformaldehyde for 20 min. before further processing. A tyramidebiotin/streptavidin amplification step was included in the in situ hybridization procedure.
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Results
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Specimen
Additional file 4 Smurf1:
embryonic day 14.5
(more  )
Specimen
Additional file 4 Smurf1:
(close  )
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Genetic Background:
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C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Fresh Frozen
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Embedding:
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Cryosection
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Analysis Tools
