Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:43626
Pelton RW, et al., Immunohistochemical localization of TGF beta 1, TGF beta 2, and TGF beta 3 in the mouse embryo: expression patterns suggest multiple roles during embryonic development. J Cell Biol. 1991 Nov;115(4):1091-105
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:3838961
|
Gene symbol:
|
Tgfb3
|
Gene name:
|
transforming growth factor, beta 3
|
Antibody:
|
anti-TGFbeta3
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to biotin
|
|
Results
|
Specimen
E12.5 skeletal system:
embryonic day 12.5
(more )
Specimen
E12.5 skeletal system:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
E13.5 skeletal system:
embryonic day 13.5
(more )
Specimen
E13.5 skeletal system:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: skeleton |
Present |
Regionally restricted |
|
High expression in chondrocytes.
|
Specimen
E14.5 skeletal system:
embryonic day 14.5
(more )
Specimen
E14.5 skeletal system:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: skeleton |
Present |
Regionally restricted |
|
High expression in chondrocytes.
|
Specimen
E15.5 skeletal system:
embryonic day 15.5
(more )
Specimen
E15.5 skeletal system:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: skeleton |
Present |
Regionally restricted |
|
High expression in chondrocytes.
|
Specimen
E16.5 skeletal system:
embryonic day 16.5
(more )
Specimen
E16.5 skeletal system:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: skeleton |
Present |
Regionally restricted |
|
Little expression in the periosteum. Expression in cytoplasm of osteocytes of bone and maturing chondrocytes such as those of tracheal cartilage.
|
Specimen
E17.5 skeletal system:
embryonic day 17.5
(more )
Specimen
E17.5 skeletal system:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: skeleton |
Present |
Regionally restricted |
|
Low levels of expression in osteogenic bone and hypertrophic cartilage.
|
TS25: tooth epithelium |
Weak |
Regionally restricted |
|
Low levels of expression in pulp cells and ameloblasts.
|
TS25: tooth mesenchyme |
Weak |
Regionally restricted |
|
Low levels of expression in pulp cells and ameloblasts.
|
TS25: tooth |
Present |
Regionally restricted |
|
No expression in the odontoblast layer.
|
Specimen
4A:
embryonic day 12.5
(more )
Specimen
4A:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
4B:
embryonic day 12.5
(more )
Specimen
4B:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
4C:
embryonic day 12.5
(more )
Specimen
4C:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: blood vessel |
Present |
Regionally restricted |
4C
|
Expression in an umbilical vessel.
|
Specimen
4D:
embryonic day 12.5
(more )
Note: Blood vessel near the spinal cord.
Specimen
4D:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Blood vessel near the spinal cord.
|
Specimen
E13.5 heart:
embryonic day 13.5
(more )
Specimen
E13.5 heart:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
E14.5 heart:
embryonic day 14.5
(more )
Specimen
E14.5 heart:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
E15.5 heart:
embryonic day 15.5
(more )
Specimen
E15.5 heart:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
E16.5 heart:
embryonic day 16.5
(more )
Specimen
E16.5 heart:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
E17.5 heart:
embryonic day 17.5
(more )
Specimen
E17.5 heart:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: heart |
Trace |
|
|
Expression barely detectable above background.
|
Specimen
E12.5 metanephros:
embryonic day 12.5
(more )
Specimen
E12.5 metanephros:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
E13.5 metanephros:
embryonic day 13.5
(more )
Specimen
E13.5 metanephros:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: renal tubule |
Present |
Regionally restricted |
|
Expression in the cuboidal epithelial lining of the metanephric tubules, but not in the basement membranes.
|
Specimen
E14.5 metanephros:
embryonic day 14.5
(more )
Specimen
E14.5 metanephros:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: renal tubule |
Present |
Regionally restricted |
|
Expression in the cuboidal epithelial lining of the metanephric tubules, but not in the basement membranes.
|
Specimen
E15.5 metanephros:
embryonic day 15.5
(more )
Specimen
E15.5 metanephros:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: renal tubule |
Present |
Regionally restricted |
|
Expression in the tubule epithelium.
|
Specimen
E17.5 metanephros:
embryonic day 17.5
(more )
Specimen
E17.5 metanephros:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: renal tubule |
Present |
Regionally restricted |
|
Decreased expression in the tubular epithelium.
|
TS25: mesenchyme of metanephros |
Present |
Regionally restricted |
|
Expression in mesenchymal cells supporting the tubules.
|
Specimen
E13.5 adrenal gland:
embryonic day 13.5
(more )
Specimen
E13.5 adrenal gland:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: gland |
Weak |
Regionally restricted |
|
Only slight expression in the adrenal gland.
|
Specimen
E14.5 adrenal gland:
embryonic day 14.5
(more )
Specimen
E14.5 adrenal gland:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: adrenal gland |
Absent |
|
|
Authors state that expression was not present in the adrenal gland during these stages of development.
|
Specimen
E15.5 adrenal gland:
embryonic day 15.5
(more )
Specimen
E15.5 adrenal gland:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: adrenal gland |
Absent |
|
|
Authors state that expression was not present in the adrenal gland during these stages of development.
|
Specimen
E16.5 adrenal gland:
embryonic day 16.5
(more )
Specimen
E16.5 adrenal gland:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: adrenal gland |
Absent |
|
|
Authors state that expression was not present in the adrenal gland during these stages of development.
|
Specimen
E17.5 adrenal gland:
embryonic day 17.5
(more )
Specimen
E17.5 adrenal gland:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: adrenal gland |
Absent |
|
|
Authors state that expression was not present in the adrenal gland during these stages of development.
|
Specimen
E14.5 intestine:
embryonic day 14.5
(more )
Specimen
E14.5 intestine:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
E15.5 intestine:
embryonic day 15.5
(more )
Specimen
E15.5 intestine:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 15.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: intestine epithelium |
Strong |
|
|
Significantly stronger expression in the intestinal epithelium.
|
Specimen
E17.5 intestine:
embryonic day 17.5
(more )
Specimen
E17.5 intestine:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: intestine epithelium |
Present |
|
|
Decreased expression compared with earlier stages.
|
TS25: intestine |
Moderate |
Regionally restricted |
|
Moderate expression in the smooth muscle of the intestine.
|
Specimen
E17.5 esophagus:
embryonic day 17.5
(more )
Specimen
E17.5 esophagus:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: esophagus |
Present |
Regionally restricted |
|
Moderate expression in the smooth muscle of the esophagus; no expression in the stratified squamous epithelium.
|
Specimen
E17.5 forestomach:
embryonic day 17.5
(more )
Specimen
E17.5 forestomach:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: stomach |
Absent |
|
|
No expression in the stratified squamous epithelium of the forestomach.
|
Specimen
E12.5 liver:
embryonic day 12.5
(more )
Specimen
E12.5 liver:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: liver |
Present |
Regionally restricted |
|
Expression in megakaryocytes.
|
Specimen
7D:
embryonic day 13.5
(more )
Specimen
7D:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: liver |
Present |
Regionally restricted |
7D
|
Expression in the liver capsule; no expression in hepatocytes.
|
TS21: liver parenchyma |
Weak |
|
7D
|
Low levels of expression in mesenchymal cells supporting the liver parenchyma.
|
Specimen
8A:
embryonic day 17.5
(more )
Specimen
8A:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
8B:
embryonic day 17.5
(more )
Specimen
8B:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: bronchiole |
Present |
Regionally restricted |
8B
|
Expression in the subepithelial region of the bronchioles.
|
TS25: lung vascular element |
Present |
Regionally restricted |
8B
|
Expression in the smooth muscle cells of blood vessels.
|
Specimen
9D:
embryonic day 13.5
(more )
Specimen
9D:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 13.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: inner ear |
Present |
Regionally restricted |
9D
|
Expression throughout the cochlear epithelium, with highest levels in the apical region.
|
Specimen
E17.5 inner ear:
embryonic day 17.5
(more )
Specimen
E17.5 inner ear:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: cochlea |
Absent |
|
|
No expression in the cochlear epithelium.
|
Specimen
10H:
embryonic day 12.5
(more )
Specimen
10H:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Present |
Regionally restricted |
10H
|
Expression in astrocytes in the ventral spinal cord.
|
Specimen
E17.5 spinal cord:
embryonic day 17.5
(more )
Specimen
E17.5 spinal cord:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: spinal cord |
Present |
Regionally restricted |
|
Expression in spinal cord astrocytes.
|
Specimen
E18.5 spinal cord:
embryonic day 18.5
(more )
Specimen
E18.5 spinal cord:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: spinal cord |
Present |
Regionally restricted |
|
Expression in spinal cord astrocytes.
|
Specimen
E14.5 skin:
embryonic day 14.5
(more )
Specimen
E14.5 skin:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS22: epidermis |
Strong |
|
|
Intense expression in the epithelial layer of the skin over the entire body.
|
Specimen
E17.5 skin:
embryonic day 17.5
(more )
Specimen
E17.5 skin:
(close )
Genetic Background:
|
involves: ICR * Swiss Webster
|
Age:
|
embryonic day 17.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
|