Gene Expression Data
Assay Details
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Assay
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Reference:
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J:113766
Zhang M, et al., Foxj1 regulates asymmetric gene expression during left-right axis patterning in mice. Biochem Biophys Res Commun. 2004 Nov 26;324(4):1413-20
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:3841413
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Gene symbol:
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Lefty2
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Gene name:
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left-right determination factor 2
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Probe:
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Lefty2 probe5
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2B:
embryonic day 7.5
(more  )
Note: This is a presomite stage embryo.
Specimen
2B:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 7.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This is a presomite stage embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo mesoderm |
Present |
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2B
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There is symmetric expression in the left and right lateral plate mesoderm.
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| TS11: node |
Absent |
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2B
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Specimen
6A:
embryonic day 8.0
(more )
Note: This is an early somite stage embryo.
Specimen
6A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This is an early somite stage embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
6A
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In 5 of 5 embryos examined there was expression in the left lateral plate mesoderm but not the right.
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Specimen
6B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Foxj1tm1Bph/Foxj1tm1Bph
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This is an early somite stage embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: embryo |
Absent |
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6B
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| TS12: lateral plate mesoderm |
Absent |
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6B
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In 10 of 10 embryos examined there was no expression in either the left or right lateral plate mesoderm.
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Specimen
6C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Foxj1tm1Bph/Foxj1tm1Bph
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This is an early somite stage embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: embryo |
Absent |
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6C
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| TS12: lateral plate mesoderm |
Absent |
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6C
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In 10 of 10 embryos examined there was no expression in either the left or right lateral plate mesoderm.
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Specimen
Not shown:
embryonic day 8.0; Foxj1tm1Bph/Foxj1 +
(more )
Note: This is an early somite stage embryo.
Specimen
Not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Foxj1tm1Bph/Foxj1+
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This is an early somite stage embryo.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
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In 15 of 15 embryos examined there was expression in the left lateral plate mesoderm but not the right.
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Analysis Tools