Probe:	NB-3 cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Bodian-fixed (80% ethanol and 10% formaldehyde). The authors did not specify which probe was used to obtain the data in Table 1, the NB-3 cDNA2 (MGI:4421766) may have been used. The data in Table 1 was obtained from brains at postnatal day 1 and and 7, no differences in expression patterns were observed between these two time points.

Structure	Level	Pattern	Image	Note
TS27: cortical layer II	Present		4A
TS27: cortical layer III	Present		4A
TS27: cortical layer V	Present		4A
TS27: cerebral cortex	Present	Regionally restricted	4A	Expression is in the subplate of the cerebral neocortex.

Structure	Level	Pattern	Image	Note
TS27: inferior colliculus	Very strong		4B
TS27: locus coeruleus	Very strong		4B
TS27: red nucleus	Very strong		4B
TS27: trigeminal nucleus	Very strong		4B

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		4C
TS28: deep cerebellar nucleus	Present		4C

Structure	Level	Pattern	Image	Note
TS27: dorsal motor nucleus of vagus nerve	Present		4D
TS27: hypoglossal nucleus	Present		4D
TS27: inferior olivary nucleus	Present		4D
TS27: trigeminal V spinal sensory nucleus	Present		4D

Structure	Level	Pattern	Image	Note
TS27: accessory olfactory bulb	Strong
TS27: anterior thalamic group	Strong
TS27: hippocampus CA1	Strong
TS27: inferior colliculus	Strong
TS27: inferior olivary nucleus	Strong
TS27: piriform cortex	Strong
TS27: trigeminal V mesencephalic nucleus	Strong
TS27: trigeminal nucleus	Strong
TS27: anterior olfactory nucleus	Present
TS27: basolateral amygdaloid nucleus	Present
TS27: cochlear nucleus	Present
TS27: cortical layer II	Present
TS27: cortical layer III	Present
TS27: cortical layer V	Present
TS27: deep cerebellar nucleus	Present
TS27: dorsal motor nucleus of vagus nerve	Present
TS27: dorsal tegmental nucleus	Present
TS27: facial nucleus	Present
TS27: hypoglossal nucleus	Present
TS27: lateral geniculate nucleus	Present
TS27: lateral reticular nucleus	Present
TS27: locus coeruleus	Present
TS27: medial geniculate nucleus	Present
TS27: parabrachial nucleus	Present
TS27: paraventricular hypothalamic nucleus	Present
TS27: red nucleus	Present
TS27: solitary tract nucleus	Present
TS27: subiculum	Present
TS27: superior colliculus	Present
TS27: superior olivary nucleus	Present
TS27: trigeminal V motor nucleus	Present
TS27: trigeminal V spinal sensory nucleus	Present
TS27: ventral posterior lateral thalamic nucleus	Present
TS27: ventral posterior medial thalamic nucleus	Present
TS27: ventromedial hypothalamic nucleus	Present
TS27: neocortex layer	Present	Regionally restricted		Expression is in the subplate.
TS27: amygdala	Present	Regionally restricted		Expression is in the dorsolateral and corticomedial nuclei.
TS27: cerebellum Purkinje cell layer	Present	Regionally restricted		Expression is in lobule 9 and 10 only.
TS27: accumbens nucleus	Absent
TS27: arcuate nucleus	Absent
TS27: caudate-putamen	Absent
TS27: cerebellum granule cell layer	Absent
TS27: cortical layer IV	Absent
TS27: cortical layer VI	Absent
TS27: dentate gyrus	Absent
TS27: dorsomedial hypothalamic nucleus	Absent
TS27: entorhinal cortex	Absent
TS27: globus pallidus	Absent
TS27: hippocampus CA3	Absent
TS27: lateral septal nucleus	Absent
TS27: main olfactory bulb external plexiform layer	Absent
TS27: main olfactory bulb glomerular layer	Absent
TS27: main olfactory bulb granule cell layer	Absent
TS27: main olfactory bulb mitral cell layer	Absent
TS27: medial septal nucleus	Absent
TS27: reticular thalamic nucleus	Absent
TS27: substantia nigra	Absent
TS27: supraoptic nucleus	Absent
TS27: ventral tegmental area	Absent

Structure	Level	Pattern	Image	Note
TS28: accessory olfactory bulb	Strong
TS28: anterior thalamic group	Strong
TS28: hippocampus CA1	Strong
TS28: inferior colliculus	Strong
TS28: inferior olivary nucleus	Strong
TS28: piriform cortex	Strong
TS28: trigeminal V mesencephalic nucleus	Strong
TS28: trigeminal nucleus	Strong
TS28: anterior olfactory nucleus	Present
TS28: basolateral amygdaloid nucleus	Present
TS28: cochlear nucleus	Present
TS28: cortical layer II	Present
TS28: cortical layer III	Present
TS28: cortical layer V	Present
TS28: deep cerebellar nucleus	Present
TS28: dorsal motor nucleus of vagus nerve	Present
TS28: dorsal tegmental nucleus	Present
TS28: facial nucleus	Present
TS28: hypoglossal nucleus	Present
TS28: lateral geniculate nucleus	Present
TS28: lateral reticular nucleus	Present
TS28: locus coeruleus	Present
TS28: medial geniculate nucleus	Present
TS28: parabrachial nucleus	Present
TS28: paraventricular hypothalamic nucleus	Present
TS28: red nucleus	Present
TS28: solitary tract nucleus	Present
TS28: subiculum	Present
TS28: superior colliculus	Present
TS28: superior olivary nucleus	Present
TS28: trigeminal V motor nucleus	Present
TS28: trigeminal V spinal sensory nucleus	Present
TS28: ventral posterior lateral thalamic nucleus	Present
TS28: ventral posterior medial thalamic nucleus	Present
TS28: ventromedial hypothalamic nucleus	Present
TS28: neocortex layer	Present	Regionally restricted		Expression is in the subplate.
TS28: amygdala	Present	Regionally restricted		Expression is in the dorsolateral and corticomedial nuclei.
TS28: cerebellum Purkinje cell layer	Present	Regionally restricted		Expression is in lobule 9 and 10 only.
TS28: accumbens nucleus	Absent
TS28: arcuate nucleus	Absent
TS28: caudate-putamen	Absent
TS28: cerebellum granule cell layer	Absent
TS28: cortical layer IV	Absent
TS28: cortical layer VI	Absent
TS28: dentate gyrus	Absent
TS28: dorsomedial hypothalamic nucleus	Absent
TS28: entorhinal cortex	Absent
TS28: globus pallidus	Absent
TS28: hippocampus CA3	Absent
TS28: lateral septal nucleus	Absent
TS28: main olfactory bulb external plexiform layer	Absent
TS28: main olfactory bulb glomerular layer	Absent
TS28: main olfactory bulb granule cell layer	Absent
TS28: main olfactory bulb mitral cell layer	Absent
TS28: medial septal nucleus	Absent
TS28: reticular thalamic nucleus	Absent
TS28: substantia nigra	Absent
TS28: supraoptic nucleus	Absent
TS28: ventral tegmental area	Absent