Probe:	Rax cDNA4
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present	Regionally restricted	S5_i	Expression is present in the hypothalamic neuroepithelium.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	S5_j	Expression is present in the hypothalamic neuroepithelium.

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present	Regionally restricted	S5_k	Expression is present in the hypothalamic neuroepithelium.

Structure	Level	Pattern	Image	Note
TS24: hypothalamus	Present	Regionally restricted	S5_l	Expression is present in the hypothalamic neuroepithelium.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present		S6_o

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	S6_p	Expression is present in the ventral hypothalamic neuroepithelium.

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present	Regionally restricted	S10_a	There is broad, selective expression in hypothalamic progenitors.

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present	Regionally restricted	S10_b	There is broad, selective expression in hypothalamic progenitors.

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present	Regionally restricted	S10_c	There is broad, selective expression in hypothalamic progenitors.

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present	Regionally restricted	S10_d	There is broad, selective expression in hypothalamic progenitors.

Structure	Level	Pattern	Image	Note
TS22: hypothalamus	Present	Regionally restricted	S10_e	There is broad, selective expression in hypothalamic progenitors.

Structure	Level	Pattern	Image	Note
TS19: diencephalon lateral wall mantle layer	Ambiguous		Rax-Shh E11.5_coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus, prethalamus, dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH, the anterior and posterior IDs, the premam, mam and supramam zones and the TT.
TS19: diencephalon lateral wall ventricular layer	Strong	Non-Uniform	Rax-Shh E11.5_coronal	There is strong staining in the VZ of the PVH, VMH & ARH nuclei, anterior ID & premam & mam zones. Staining is either absent or ubiquitous in the VZ of the dorsal thalamus, prethalamus, dorsal & ventral POAs, the AVH, posterior ID, TT & supramam zone.
TS19: telencephalon mantle layer	Ambiguous		Rax-Shh E11.5_coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone and cortical plate of the cerebral cortex and in the mantle zone of the ganglionic eminences.
TS19: telencephalon ventricular layer	Ambiguous		Rax-Shh E11.5_coronal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex and the ganglionic eminences.

Structure	Level	Pattern	Image	Note
TS20: cerebral cortex intermediate zone	Ambiguous		Rax-Shh E12.5_coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS20: cerebral cortex ventricular layer	Ambiguous		Rax-Shh E12.5_coronal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS20: cortical plate	Ambiguous		Rax-Shh E12.5_coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS20: diencephalon mantle layer	Ambiguous		Rax-Shh E12.5_coronal	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS20: diencephalon ventricular layer	Strong	Non-Uniform	Rax-Shh E12.5_coronal	There is strong staining in the ventricular zone of the mam zone. Staining is either absent or ubiquitous in the ventricular zone of the premam and supramam zones and the TT.
TS20: ganglionic eminence	Ambiguous		Rax-Shh E12.5_coronal	Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS20: hypothalamus mantle layer	Ambiguous		Rax-Shh E12.5_coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS20: hypothalamus ventricular layer	Strong	Non-Uniform	Rax-Shh E12.5_coronal	There is strong staining in the ventricular zone of the PVH, VMH and ARH nuclei and the anterior ID. Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the AVH and posterior ID.
TS20: thalamus mantle layer	Ambiguous		Rax-Shh E12.5_coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS20: thalamus ventricular layer	Ambiguous		Rax-Shh E12.5_coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Structure	Level	Pattern	Image	Note
TS22: cerebral cortex intermediate zone	Ambiguous		Rax-Shh E14.5_coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS22: cerebral cortex ventricular layer	Ambiguous		Rax-Shh E14.5_coronal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS22: cortical plate	Ambiguous		Rax-Shh E14.5_coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS22: diencephalon mantle layer	Ambiguous		Rax-Shh E14.5_coronal	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones and the TT.
TS22: diencephalon ventricular layer	Strong	Non-Uniform	Rax-Shh E14.5_coronal	There is strong staining in the ventricular zone of the premam and mam zones. Staining is either absent or ubiquitous in the ventricular zone of the TT and the supramam zones.
TS22: ganglionic eminence	Ambiguous		Rax-Shh E14.5_coronal	Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS22: hypothalamus mantle layer	Ambiguous		Rax-Shh E14.5_coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, VMH and ARH nuclei, the AVH and anterior and posterior IDs.
TS22: hypothalamus ventricular layer	Strong	Non-Uniform	Rax-Shh E14.5_coronal	There is strong staining in the ventricular zone of the anterior ID and VMH and ARH nuclei and moderate staining in the PVH nucleus. Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the AVH and posterior ID.
TS22: thalamus mantle layer	Ambiguous		Rax-Shh E14.5_coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS22: thalamus ventricular layer	Ambiguous		Rax-Shh E14.5_coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex intermediate zone	Ambiguous		Rax-Shh E16.5_coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the intermediate zone of the cerebral cortex.
TS24: cerebral cortex ventricular layer	Ambiguous		Rax-Shh E16.5_coronal	Staining is either absent or ubiquitous in the ventricular zone of the cerebral cortex.
TS24: cortical plate	Ambiguous		Rax-Shh E16.5_coronal	Staining is either absent or ubiquitous in the postmitotic neurons in the cortical plate of the cerebral cortex.
TS24: diencephalon mantle layer	Ambiguous		Rax-Shh E16.5_coronal	Staining is either absent or ubiquitous in the mantle zone of the premam, mam and supramam zones.
TS24: diencephalon ventricular layer	Strong	Non-Uniform	Rax-Shh E16.5_coronal	There is strong staining in the ventricular zone of the premam and mam zones. Staining is either absent or ubiquitous in the ventricular zone of the supramam zone.
TS24: ganglionic eminence	Ambiguous		Rax-Shh E16.5_coronal	Staining is either absent or ubiquitous in the ventricular and mantle zones of the ganglionic eminences.
TS24: hypothalamus mantle layer	Ambiguous		Rax-Shh E16.5_coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal and ventral POAs, the PVH, SCH, DMH, VMH and ARH nuclei, the AVH, the DAH and the PH.
TS24: hypothalamus ventricular layer	Strong	Non-Uniform	Rax-Shh E16.5_coronal	There is strong staining in the ventricular zone of the PVH, DMH, VMH and ARH nuclei, the DAH and the PH. Staining is either absent or ubiquitous in the ventricular zone of the dorsal and ventral POAs, the AVH and the SCH nucleus.
TS24: thalamus mantle layer	Ambiguous		Rax-Shh E16.5_coronal	Staining is either absent or ubiquitous in the mantle zone of the dorsal thalamus and prethalamus.
TS24: thalamus ventricular layer	Ambiguous		Rax-Shh E16.5_coronal	Staining is either absent or ubiquitous in the ventricular zone of the dorsal thalamus and prethalamus.